河北省脑膜炎奈瑟菌抗生素敏感性监测

Neisseria meningitidis antibiotic susceptibility monitoring of isolates from Hebei Province

目的了解河北省2013年收集到的42株脑膜炎奈瑟菌(Neisseria meningitidis,Nm)药物敏感性。方法根据美国临床实验室标准化研究所(CLSI/NCCLS)推荐,选择治疗类和预防类抗生素共12种进行K-B纸片法或E-test试纸条法药敏试验。结果 42株菌株对美罗培南、氯霉素、阿奇霉素、利福平、米诺环素全敏感,对磺胺甲基异恶唑全耐药。青霉素和氨苄西林敏感率仅59.52%和54.76%,环丙沙星和萘啶酸的耐药率分别为80.95%和90.48%。发现1株健康人员来源的B群Nm对青霉素耐药,1株病人来源的B群Nm对头孢噻肟和头孢曲松均不敏感。结论在该省首次发现对青霉素耐药菌株及头孢类药物不敏感菌株。结合历年监测结果,由于耐药率较高,预防性用药时不推荐使用磺胺类和喹诺酮类抗生素。

Objective To study the drug susceptibility of 42 Neisseria meningitidis （Nm） isolated from Hebei province. Methods According to the latest CLSI/NCCLS, K - B assay and MIC assay were carried to test the susceptibility of 12 drugs of treatment and prevention. Results All the 42 Nm were susceptible to meropenem, chloramphenicol, azithromycin, rifampicin, minocycline, and all isolates were resistant to sulfamethoxazole. The susceptible rate of penicillin and ampicillin were 59. 52% and 54. 76%. The resistant rate of ciprofloxacin and nalidixic acid were 80. 95% and 90.48%. There was 1 strain of serum group B of Nm isolated from healthy people resistant to penicillin, and 1 strain of serum group B of Nm isolated from patient not susceptible to cefotaxime and ceftriaxone. Conclusions For the first time in Hebei province, found the strains resistant to penicillin and not susceptible to cephalosporins. Combining historical monitoring results, sulfa drugs and quinolones drugs are not recommended for epidemic cerebrospinal meningitis prevention because of the high resistant rate.