衡阳地区春夏季婴幼儿上呼吸道感染性疾病病毒谱分析

Investigation of respiratory tract infections in infants and young children during spring and summer in Hengyang

目的：了解衡阳地区春夏两季婴幼儿上呼吸道病毒感染的流行状况。方法收集2013年2～8月门诊和住院患儿咽拭子855例，其中春季（2～5月）468例，夏季（6～8月）387例；分别采用荧光抗体检测副流感病毒1、副流感病毒2、副流感病毒3，流感病毒A、流感病毒B、呼吸道合胞病毒、腺病毒，采用实时荧光PCR定量（RT-PCR）检测EB病毒。结果病毒检出率35.8%（306/855），副流感病毒1春季1.3%（6/468），夏季6.7%（26/387），差异有显著性（χ2=17.4，P＜0.01）；副流感病毒2春季0.65%（3/468），夏季2.2%（9/387），差异无显著性（χ2=4.3，P＞0.05）；副流感病毒3春季1.3%（6/468），夏季4.6%（18/387），差异有统计学意义（χ2=8.8，P＜0.05）；流感病毒（ A、B）春季均为0.65%（3/468），夏季均未检出，差异无统计学意义（χ2=2.5，P＞0.05）；呼吸道合胞病毒（ RSV），春季2.2%（10/458），夏季0.3%（1/386）（χ2=5.9，P ＜0.05）；腺病毒（ADV），春季5.1%（24/468），夏季13.2%（51/387），差异有统计学意义（χ2=17.1，P＜0.01）；EB病毒，春季16.9%（79/468），夏季17.3%（67/387），差异无统计学意义（χ2=0.02，P＞0.05）；同时检出腺病毒和EB病毒共57例检出率为6.7%（57/855）。结论 EB病毒、腺病毒、副流感病毒（1、2、3）型是衡阳地区患儿上呼吸道感染的主要病原体，除EB病毒外其他病毒流行有一定季节性。

Objective To investigation of respiratory tract infections in infants and young children during spring and summer in Hengyang. Methods 855 samples were collected from nasopharyngeal swabs of infants of clinic service and hospi-talization in our hospital during 2～8 month,2013. And 468 from Spring（2～5 month）,387 from Summer（6～8 month）. The virus were detected by using direct fluorescent antibody（DFA）;Parainfluenza Virus 1,2 and 3,Influenza Virus A and B, Respiratory Syncytial Virus,Adenovirus,EBV was determined by RT-PCR. Results The virus were observed in 306 out of 855 cases（35. 8%）,the rates of between Spring and Summer are 28. 6%,44. 4%,respectively（P〈0. 05）. And the incidence is 1. 3%,0. 65%,1. 3%,0. 65%,0. 65%,5. 1%,2. 1%,16. 9% and 6. 7%,2. 2%,4. 6%,0%,0%,13. 2%,0. 3% 17. 3%among Parainflu 1,2 and 3,influenza A and B,ADV,RSV,EBV between Spring and Summer in Hengyang city,respectively. There are significantly different（P〈0. 05）for Parainflu 1 and 3,ADV and RSV in Spring and Summer and others are not sig-nificantly different（P〉0. 05）. The co-infection of ADV and EBV was found in 57 out of 855 cases（6. 7%）. Conclusion The predominant prevalence of respiratory virus are EBV,ADV and Parainfluenza Virus（1,2,3）for infants of upper respirato-ry tract infection of virus in Hengyang city in China. There are seasonal differencing for those virus but for EBV.