肺炎支原体血清学被动凝集法与胶体金法检测阳性率差异

Application of passive particle agglutination and colloidal gold method for mycoplasma pneumonia in children

目的：探讨肺炎支原体血清学被动凝集法与胶体金法在小儿肺炎支原体肺炎疾病检测中阳性率差异。方法随机选取2014年1～12月儿科肺炎患儿108例，在病程7～10d内抽取静脉血，分离血清，同时采用被动凝集法与胶体金法进行支原体抗体检测，比较被动凝集法与胶体金法检测阳性率的差异。结果108例患儿中，肺炎支原体血清学被动凝集法与胶体金法阳性率分别为26.3%和15.6%（P＜0.05）。结论在小儿肺炎支原体肺炎疾病的临床诊疗中，血清学被动凝集法检出率更高。

Objective To explore the significance of applying passive particle agglutination（ PPA）and colloidal gold method（ GICA）in children with mycoplasma pneumonia（ MP）. Methods MP in 108 children with Mycoplasma pneumoniae pneumonia within 7～10 days of the disease course was examined with PPA and GICA. MP in the team was rechecked by these two methods between 7 to 10 days of the disease course and positive rates、sensitivities、specificities were compared. Results The results of PPA and GICA examinations within 5～7 days of the disease in 108 cases showed that the positive rates of MP were 26. 3% and 15. 6%（P〈0. 05）. Conclusion Examinations of MP with PPA methods are prefer to GICA in diagnosis of mycoplasma pneumoniae pneumonia.