降钙素相关肽基因多态与1型糖尿病的关系研究

The relationship between polymorphisms of Human beta-CGRP gene exon 4 for calcitonin-like peptide and type 1 diabetes

目的探讨降钙素相关肽基因(calcitonin gene related peptide,CGRP)多态及其血清CGRP相关肽水平与1型糖尿病发生发展的关系。方法采用聚合酶链反应(polymerase chain reaction,PCR)技术,对42例1型糖尿病患者和126例正常对照的降钙素相关肽基因进行全长扩增并进行基因型分析,统计1型糖尿病组和正常对照组的CGRP主要的基因型基因频率,同时采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定各组别各基因型血清降钙素基因相关肽浓度的差异。结果单变量Logistic回归分析显示类降钙素基因多态性(155209 C)与1型糖尿病发生的风险相关,相关系数为3.27,1型糖尿病组患者的血清CGRP浓度为正常对照组1.40倍。结论降钙素基因多态性与1型糖尿病遗传易感性相关,血清CGRP浓度可以作为1型糖尿病的新型标志物。

Objective：Discussion of β calcitonin gene（Human beta-CGRP gene exon 4 for calcitonin-like peptide,CGRP）the relationship between polymorphism and serum CGRP peptide level and the development of type 1 diabetes. Methods：Polymerase chain reaction（polymerase chain reaction,PCR）,42 cases of type 1diabetic patients and 126 normal control class β calcitonin gene full-length amplification and genotyping,gene frequency statistics of type 1 diabetes group and normal control group beta calcitonin gene type,at the same time enzyme linked immunosorbent assay（enzyme-linked immunosorbent assay,ELISA）were determined by difference of calcitonin gene related peptide concentration in each category of each genotype serum. Results：Univariate Logistic regression analysis showed that beta calcitonin gene polymorphism（155209 C）are associated with the risk of type 1 diabetes,the correlation coefficients were 3.27,the concentration of serum CGRP of patients with type 1diabetes with normal control group 1.40 times. Conclusions：Calcitonin gene polymorphism and genetic susceptibility to type 1diabetes,serum CGRP concentration can be used as a novel marker for type 1 diabetes.