体外受精-胚胎移植中卵丘颗粒细胞mtDNA4977bp缺失的研究

Research of the deletion of mitochondria DNA 4977bp of cumulus granulosa cells in IVF-ET

目的探讨高龄患者卵丘颗粒细胞mt DNA结构的完整性,为卵丘颗粒细胞mt DNA结构的完整性在辅助生殖技术中的应用提供理论依据。方法将接受体外受精-胚胎移植(IVF-ET)治疗的72例女性患者分为高龄组(年龄≥38岁)和非高龄组(年龄<38岁),收集其卵丘颗粒细胞,应用PCR方法检测mt DNA4977bp的缺失情况。结果高龄组患者的获卵数、优胚率及妊娠率均明显低于非高龄组(P<0.05)。两组患者均未检测到卵丘颗粒细胞mt DNA4977bp的缺失。结论接受IVF-ET治疗的女性患者,无论高龄与否,卵丘颗粒细胞mt DNA结构基本完整。

Objective：To research the structure integrity of Mitochondria DNA in cumulus granular cells of elderly patients,so as to provide theoretical evidence to the application of it in IVF-ET. Methods：72 women were divided into two groups：elder group（age≥38 years）and non-elder group（age〈38 years）. Collected their cumulus granulosa cells,and detected the deletion of mitochondria DNA 4977 bp by PCR. Results：The number of oocytes retrieved,high quality embryo rate and pregnancy rate were obviously lower in elder patients than those in non-elder patients（P〈0.05）. There was no deletion of mitochondria DNA4977 bp in the two groups. Conclusions：The structure of mitochondria DNA in cumulus granulosa cells of the patients whether elder or not is integrited in IVF-ET.