HPLC法同时测定肝力保胶囊中绿原酸及虎杖苷的含量

Simultaneous Determination of Chlorogenic Acid and Polydatin in Ganlibao Capsules by HPLC

目的建立HPLC法同时测定肝力保胶囊中绿原酸和虎杖苷的含量。方法色谱柱为Agilent ZORBAX SBC18,流动相为0.1%甲酸-乙腈梯度洗脱,流速：1.0 ml·min-1,柱温：25℃,检测波长：327 nm,进样量5μl。结果绿原酸在1.365～68.25μg·ml-1（r=1.000）范围内线性关系良好,虎杖苷4.00～200.00μg·ml-1（r=1.000）范围内线性关系良好;日内和日间精密度均小于2%（n=3）;绿原酸平均回收率为100.81%（RSD=1.74%,n=6）,虎杖苷平均回收率99.87%（RSD=1.30%,n=6）。结论该法简便、结果准确、重复性好,可用于肝力保胶囊中绿原酸和虎杖苷的质量控制。

Objective To establish an HPLC method for determination of chlorogenic acid and polydatin in Ganlibao capsules. Methods Agilent ZORBAX SB-C18 column was used, the moble phase was 0.1% formic acid in acetonitrile at a flow rate of 1.0 ml ·min- 1, the temperature was at 25 ℃, the detection wavelength was 327 nm, and the injection volume was 5 μl. Results The calibration curve was linear for chlorogenic acid within the range of 1. 365-68.25 μg ·ml-1 （ r = 1. 000）,and 4.00-200.00 μg ·ml-1 （ r = 1. 000） for polydatin. The results of intra-day and inter-day precision were both within 2% （ n = 3）. The average recovery was 100. 81% （RSD = 1.74% , n = 6） and 99.87% （ RSD = 1.30% , n = 6 ）, respectively. Conclusion This method is simple, accurate, and with satisfactory repeatability, which can be used for quality control of chlorogenic acid and polydatin in Ganlibao capsules.