微生物谷氨酰胺转氨酶的优势结构及构效特征研究进展

Advantageous Structure and Characteristic of Structure-activity in Microbial Transgutaminase

微生物谷氨酰胺转氨酶可催化蛋白质分子间和分子内发生共价交联反应,由于其具有非Ca2+依赖性,底物特异性低,催化速度快,生产成本低等优势,因此被广泛地应用于食品、医药及纺织等领域。本文介绍了微生物谷氨酰胺转氨酶的理化性质、构象优势,并对微生物谷氨酰胺转氨酶的底物肽序列特征,结构中与催化作用相关的氨基酸残基及其分子改造等前瞻性研究进行综述。

Microbial transglutaminase could incorporate inter-or intramolecular covalent cross-links. Due to its Ca^2＋- independence, broad substrate specificities, rapid reaction rate and low production cost, it has been widely used in food, medicine and textile industry. In this article, enzymatic properties and advantageous structure of microbial transglutaminase were reviewed, especially preferred substrate sequences, amino acid residues relate with enzymatic activity and molecular modification of microbial transglutaminase were briefly summaried and analyzed.