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环介导等温扩增技术的假阳性扩增研究 被引量：4

Study on False-positive Amplification of Loop-mediated Isothermal Amplification
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摘要 将环介导等温扩增技术最初报道中的M13噬菌体引物排列成四种组合FIP+BIP+F3+B3、FIP+BIP+F3、FIP+BIP+B3、FIP+BIP,分别在不加M13噬菌体模板的情况下以相同的反应体系及反应条件进行等温扩增,琼脂糖凝胶电泳检测扩增产物,分析环介导等温扩增中产生假阳性的原因.结果表明,关于环介导等温扩增技术最初报道的试验具有非特异性扩增现象,环介导等温扩增的假阳性主要是引物二聚体引起的而并非是气溶胶污染导致的. The primers for M13 phage in the initial report of Loop-mediated Isothermal Amplification （LAMP） were divided into 4 combinations： FIP ＋ BIP ＋ F3 ＋ B3, FIP ＋ BIP ＋ F3, FIP ＋ BIP ＋ B3 and FIP ＋ BIP, the isothermal amplification was performed with the same reaction system and reaction eonditions, respectively, no M13 phage DNA template was added to any of these reactions, the amplified products were deteeted with agarose gel eleetrophoresis, and the reasons of false positive occurred in LAMP were analyzed. The results indieated that the primers initially reported amplified non-specifically, the false positive amplification of LAMP was mainly due to primer dimers rather than aerosol pollution.
作者 王德国 王永真 王爱萍
机构地区 许昌学院食品与生物工程学院河南省博士后研发基地 许昌学院公共实验中心 郑州大学生命科学学院
出处 《许昌学院学报》 CAS 2015年第5期81-83,共3页 Journal of Xuchang University
基金 NSFC-河南人才培养联合基金(U1204330) 国家自然基金面上项目(31172331) 河南省高等学校科技创新团队支持计划(15IRTSTHN016) 河南省高等学校青年骨干教师资助计划项目(2012GGJS-172) 许昌学院杰出青年骨干人才培养计划项目
关键词 脱氧核糖核酸 环介导等温扩增 假阳性 非特异性扩增 deoxyribonucleic acid loop-mediated isothermal amplification, false positive nonspecific amplification
分类号 Q81 [生物学—生物工程]
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参考文献17

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二级参考文献13

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共引文献22

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许昌学院学报
2015年 第5期

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