二倍体与四倍体苹果的三倍性杂交后代ISSR遗传分析

Genetic Analysis of Apple Triploid Hybrid Progenies from Diploid and Tetraploid Based on ISSR

利用ISSR分子标记技术对苹果‘寒富’与‘四倍体嘎拉’杂交的三倍性后代及亲本进行遗传变异研究。结果表明9条ISSR引物共扩增出多态性条带58条,多态性比率为67.4%。相似性分析可知,三倍性新种质与母体相似系数在0.5909~0.7692之间,与父本相似系数在0.5714~0.7500之间,聚类结果显示三倍性杂交后代具有母本倾向。

The genetic variation of triploid hybrid progenies from ‘Hanfu’ב4 x Gala’were studied based on ISSR molecular markers in this paper.The results showed that 58 polymorphic bands were amplified by 9 ISSR primers,the percentage of polymorphic bands of ISSR was 67.4%.The genetic similarity was 0.5909 ~0.7692 between triploid hybrid progenies and female parent,and it was 0.5714 ~0.7500 between triploid hybrid progenies and male parent.The cluster result showed that most of triploid hybrid progenies were tend to the female parent ‘Hanfu’.