大垫尖翅蝗转录组分析

An analysis of the transcriptome of Epacromius coerulipes( Orthoptera:Acrididae)

【目的】大垫尖翅蝗Epacromius coerulipes(Ivanov)是广泛分布的草原蝗虫之一,但其基因资源缺乏。为了获得大垫尖翅蝗的基因数据,对其进行了转录组测序和分析。【方法】利用Illumina公司paired-end转录组的测序技术进行从头组装。【结果】总计获得了63 033条unigenes,平均长度为772 bp,N50为1 589 bp。通过BLAST搜索,确定有25 132条(39.87%)unigenes与NCBI数据库已知的蛋白质相匹配,其中有24 841,16 490,11 558和8 013条unigenes成功注释到Nr,Swiss-Prot,GO和COG数据库中。KEGG数据库中,7 218条unigenes形成218条代谢或信息通路。其中,189条unigenes参与外源性物质或药物的代谢通路。进一步分析显示,213条unigenes被确认为可能参与外源性物质的解毒作用,29条unigenes被确定为编码杀虫剂的目标蛋白。此外,检测到5 696条简单重复序列。【结论】该转录组测序分析将为进一步研究大垫尖翅蝗的基因功能分析及杀虫剂的抗药性机制分析奠定分子基础。

[Aim]Epacromius coerulipes（ Ivanov） is one of the most widely distributed grassland locusts.However,there is a lack of genomic resources. In order to obtain genetic data of E. coerulipes,this study conducted the transcriptome sequencing and analysis. [Methods]De novo assembly of the transcriptome was conducted using Illumina paired-end sequencing technology. [Results]In total,63 033 unigenes with a mean length of 772 bp and an N50 length of 1 589 bp were obtained. Of these unigenes,25 132（ 39. 87%） unigenes were matched with the known proteins in the National Center for Biotechnology Information（ NCBI） database,as determined by BLAST search,with 24 841,16 490,11 558 and 8 013 unigenes assigned to Nr database,Swiss-Prot,Gene Ontology（ GO） and Clusters of Orthologous Groups（ COG）,respectively. In KEGG database,a total of 7 218 unigenes were assigned to 218 known pathways. Among these,189 unigenes were involved in metabolic pathways of xenobiotics or drugs. Indepth analysis of the data showed that 213 unigenes were identified as potentially involved in the detoxification of xenobiotics,and 29 unigenes were identified as encoding insecticide target proteins. In addition,5 696 simple sequence repeats（ SSRs） were detected. [Conclusion ] This study lays the foundation for further research on gene function analysis and molecular resistance mechanisms to insecticides in E. coerulipes.