超声辅助提取发酵豆渣蛋白工艺研究

Ultrasound-assisted extraction technology of protein from fermented okara

以豆渣为原料,首先采用黑曲霉(Aspergillus niger)发酵豆渣,然后分别应用超声辅助碱溶酸沉法和单一碱溶酸沉法提取已发酵豆渣中的蛋白质。单因素和正交试验结果表明:超声辅助碱溶酸沉法豆渣蛋白提取率明显升高,提取时间也大大缩短;最佳工艺参数为碱提温度55℃,提取时间90 min,碱液浓度0.3 mol/L,超声功率300 W,料液比1∶24(g∶m L)。在此最佳条件下,豆渣蛋白提取率是83.84%。

Using okara as raw material, Aspergillus niger as starter to ferment okara, and then protein was extracted from fermented okara by ultra- sound-assisted alkali solution acid precipitation method and single alkali solution acid precipitation method. The single factor and orthogonal experi- ment results showed that ultrasound-assisted alkali solution acid precipitation method was better than single alkali solution acid precipitation in in- creasing okara extraction rate and reducing the extraction time greatly. The optimal condition were as follows： ultrasound temperature 55 ℃, alkali extraction time 90 min, NaOH 0.3 mol/L, ultrasonic power 300 W, solid-liquid ratio 1：24 （g：ml）. Under this condition, the optimal okara protein ex- traction rate was 83.84%.