基于因素最少化的槟榔多酚错流提取条件研究

Cross-flow extraction conditions of Areca catechu polyphenol based on the least factor

有效提高槟榔多酚提取率,采用多种溶剂体系进行提取,探讨最佳提取溶剂。根据错流浸取工艺理论设计了料液比与浸取级数,并在单因素试验的基础上进行正交优化设计,最终确定了槟榔中多酚提取的最佳工艺条件。结果表明,最佳提取剂为混合溶剂(甲醇∶丙酮∶水=1∶2∶2)(V∶V),料液比为1:20(g:m L),p H值为4.0,在70℃下提取100 min,提取次数为2,槟榔多酚提取率达到34.46 mg/g。

In order to improve the polyphenol extraction rate, Areca catechu polyphenol was extracted by different extraction solvent systems to ex- plore the optimal extraction solvent. The material/liquid ratio and extractive grades were designed based on the theory of multistage cross flow extrac- tion process. In addition, on the basis of single factors test, the optimal polyphenol extraction condition was determined by orthogonal test. The results showed that the optimal conditions for the extraction of A. catechu polypbenol were as follows： mixed solvent （methanol：acetone：wate =1：2：2） （V： V） as extraction solvent, material/liquid ratio 1：20 （g：ml）, pH 4.0, extraction time 100 min, temperature 70 ℃, extraction times 2. Under this condition, the extraction efficiency was 34.46 mg/g.