AaeDV编码的miRNA样分子的分析与鉴定

Identification of MicroRNA-like Viral Small RNAs from AaeDV

一些双链DNA病毒和RNA病毒可以在宿主细胞内编码自身的miRNAs分子,其在病毒感染宿主细胞与病毒复制过程中发挥重要的作用,为研究单链DNA病毒是否可编码miRNAs,我们利用VMir、miRNAFold和MaturePred软件,通过生物信息学方法预测了一株单链DNA病毒-埃及伊蚊浓核病毒(Aedes aegypti densovirus,AaeDV)编码的miRNAs分子的颈环结构前体与成熟体,并通过Northern blot与stem-loop RT-PCR方法进行了验证,结果在预测的4个茎环结构前体中,通过stem-loop RT-PCR方法验证到一个miRNA样分子AaeDVMD。该结果证实了单链DNA病毒可编码miRNAs,为进一步研究蚊浓核病毒与宿主蚊类之间的相互作用提供了新的研究方向。

Several virus families have been shown to encode microRNAs （miRNAs）, which have roles in the infection and replication of viruses in host cells. These virus-encoded miRNAs are identified in double- stranded DNA virus （dsDNA virus） and in several RNA virus families, but not in single-stranded DNA vi- rus （ssDNA virus）. We used a bioinformatics approach based on VMir, miRNAFold and MaturePred software to predict virus-encoded miRNA-like small RNAs from the genome of a ssDNA virus： Aedes ae- gypti densovirus （AaeDV）. Northern blotting and stem-loop reverse transcription-polymerase chain reac- tion （RT-PCR） were used to detect predicted small RNAs. A miRNA-like small RNA termed ＂AaeD- VMD＂ was identified by stem-loop RT-PCR from predicted candidates. This is the first report demonstra- ting that a ssDNA virus can encode miRNA-like small RNAs. These data will aid further exploration of the interaction between the AaeDV and its mosquito host.