应用二代测序技术研究HIV准种进化的相关进展

Application of Next-generation Sequencing Techniques in the Dynamics of HIV-1 Quasispecies

近十年二代测序技术快速发展,与一代测序技术相比具有通量高、速度快、灵敏度高、准确率高等优点,主要以454焦磷酸测序、Illumina测序、Ion Torrent测序和SOLiD测序为代表。二代测序技术在HIV领域的研究已经越来越广泛,本文介绍了上述四种二代测序技术在HIV准种进化方面的应用。

In the last decade, next-generation sequencing （NGS） technology, which is characterized by be- ing high-throughput, rapid, sensitive, and accurate, has developed rapidly. Main components of NGS are platforms： 454 sequencing; illumina sequencing; ion torrent sequencing~ SOLID sequencing. NGS is used widely for the human immunodeficiency virus （HIV）-I. In this review, we focus on applications of the dy- namics of HIV-1 quasispecies.