HPLC同时测定甘薯茎叶中叶黄素和β–胡萝卜素

HPLC analysis method on Lutein and β-carotene in sweet potato(Ipomoea batatas Lam) leaves

以水果薯、水果花叶薯、紫羽1号、紫羽2号、农大紫1号、农大紫2号、浙紫1号、紫薯2号、高淀粉、叶用薯1号、叶用薯2号等11种不同品种甘薯茎叶为试验材料,建立了高效液相色谱法同时测定甘薯茎叶中叶黄素和β–胡萝卜素含量的方法。结果表明：采用甲醇为提取溶剂,料液比1∶30,50℃超声提取3次,每次40 min,能较完全提取甘薯茎叶中的叶黄素和β–胡萝卜素;HPLC以甲醇–水（95∶5）、甲醇–乙腈–四氢呋喃（70∶20∶5）为流动相梯度洗脱,叶黄素和β–胡萝卜素分离良好,回收率分别为105.8%和101.5%;叶黄素及β–胡萝卜素含量最高的是甘薯茎叶中的成熟叶,其次为新叶,茎的含量最低;成熟叶中叶黄素与β–胡萝卜素的含量分别在0.504~1.031 mg/g和0.188~0.467 mg/g,新叶中叶黄素与β–胡萝卜素的含量分别在0.409~0.617 mg/g和0.141~0.242mg/g,叶柄中叶黄素与β–胡萝卜素的含量分别在0.094~0.195 mg/g和0.040~0.082 mg/g,茎中叶黄素与β–胡萝卜素的含量分别在0.053~0.103 mg/g和0.036~0.045 mg/g。

Stems and leaves of 11 sweet potato varieties（ Shuiguo, Shuiguo-huaye, Ziyu 1, Ziyu 2, Nongda-zi 1, Nongdazi 2, Zhezi 1, Zishu 2, Gaodian-fen, Yeyong 1 and Yeyong 2） were used as material, to study the method of HPLC for the determination of lutein and β-carotene simultaneously. The results showed that lutein and β-carotene could be extracted from sweet potato leaves by ultrasound extracted with 1∶30 of materials to methanol at 50 ℃ three times, each time with 40 min. With HPLC mobile phase of methanol-water（95∶5, v/v） and methanol-acetonitrile-tetrahydrofuran（70∶20∶5）, lutein and β-carotene could be separated well and their recoverys were 105.8% and 101.5% respectively. Content of lutein and β-carotene in 11 varieties were the highest in mature leaves（0.504–1.031 mg/g and 0.188–0.467 mg/g）, then the tender leaves（0.409–0.617 mg/g and 0.141–0.242 mg/g） and petioles（0.094–0.195 mg/g and 0.040–0.082 mg/g） the lowest levels in stems（0.053–0.103 mg/g and 0.036–0.045 mg/g）.