摘要
目的了解南宁市婴幼儿散发性腹泻病例中诺如病毒（Norovirus,NV）基因Ⅱ组（GenogroupⅡ,GⅡ）4型变异株的组成和流行特点。方法采集2010年1月-2011年12月南宁市某医院门诊354例婴幼儿腹泻病例的粪便标本,应用实时荧光逆转录-聚合酶链反应检测NV核酸及其基因组,阳性标本进行核苷酸序列测定和遗传进化分析。结果 354份标本中,NV核酸检测阳性101份,阳性率28.5%,全部属于GⅡ。对测序成功的84株NV的核苷酸序列进行分析,77.4%属于GⅡ.4型（65株）,其余为GⅡ.2型（7株）、GⅡ.14型（4株）、GⅡ.7型（3株）、GⅡ.3型（2株）、GⅡ.6型（2株）和GⅡ.12型（1株）。65株GⅡ.4型中,60株（92.3%）为2006b变异株,5株（7.7%）为2010变异株。结论 GⅡ.4型是南宁市婴幼儿中NV所致散发性腹泻的最主要基因型,其中2006b变异株占优势。首次在本地区检测到2010变异株,且该毒株的流行呈上升趋势。有必要加强监测,以及时掌握GⅡ.4型变异株的流行变迁。
Objective To gain insight into the epidemiologic pattern of genotype GⅡ. 4 variants of noroviruses（ NVs） in sporadic cases with acute gastroenteritis among children in Nanning city. Methods A total of 354 stool specimens were collected from children presenting with diarrhea at an outpatient clinic of a hospital in Nanning city,from January 2010 to December 2011. Specimens were screened for the presence of NV ribonucleic acid（ RNA） by one-step,real-time reverse transcription-polymerase chain reaction（ RT-PCR）. NV-positive strains were subsequently sequenced for phylogenetic analysis. Results A total of 101（ 28. 5%） of 354 specimens were positive for NV RNA,and all belonged to genogroupⅡ（ GⅡ）. Eighty-four strains were successfully sequenced. Phylogenetic analysis showed that the most commonly identified genotype was GⅡ. 4（ 65 strains,77. 4%）,followed by GⅡ. 2（ 7 strains）,GⅡ. 14（ 4 strains）,GⅡ. 7（ 3 strains）,GⅡ. 3（ 2 strains）,GⅡ. 6（ 2 strains） and GⅡ. 12（ 1 strain）. The GⅡ. 4 strains included two variants,GⅡ. 4 2006b（ 60 /65,92. 3%） and GⅡ. 4 2010（ 5 /65,7. 7%）.Conclusion GⅡ. 4 is the dominant genotype of NV-associated sporadic gastroenteritis among children in Nanning city,with the variant GⅡ. 4 2006 b circulating predominantly. The variant GⅡ. 4 2010 was first detected in Nanning and showed an increased incidence. The study findings indicate the need in continuous surveillance to monitor epidemiologic changes of GⅡ. 4 variants.
出处
《中国疫苗和免疫》
CAS
北大核心
2015年第2期190-195,共6页
Chinese Journal of Vaccines and Immunization
基金
广西壮族自治区卫生厅科学研究课题(合同号:S201310-05)
