IL-17及其受体调控JAK/STAT3信号通路促进喉癌血管生成的作用

The role of IL-17 and its receptor in invasion and metastasis of laryngeal cancer

目的探讨白细胞介素17(IL-17)及其受体调控酪氨酸激酶(JAK)/转录激活因子-3(STAT3)信号通路促进喉癌血管生成的作用。方法收集喉癌手术切除标本70例,癌组织通过不同病理分级,采取70例喉癌患者的外周血。采用ELISA法检测外周血中IL-17、血管内皮生长因子A(VEGF-A)的浓度,分析其与临床病理特征的关系。Western blot法检测喉癌组织中IL-17、IL-17受体(IL-17R)、JAK1/2、p-JAK1/2、STAT3、p-STAT3、VEGF-A、环氧合酶-2(COX-2)、基质金属蛋白酶-9(MMP-9)等信号分子水平的变化。结果喉癌患者血清中IL-17、VEGF-A的含量与喉癌的分化程度、淋巴结有无转移和TNM分期有关(P<0.05),Western blot结果显示在喉癌发展的不同时期,随着喉癌的恶化,IL-17、IL-17R的表达逐渐增强,相关信号通路分子JAK1/2、pJAK1/2、STAT3、p-STAT3、VEGF-A、COX-2、MMP-9表达也显著增加。结论 IL-17可能调控JAK/STAT3信号通路,在喉癌的血管生成中起到重要的作用。

Objective To investigate IL-17 and its receptor regulate JAK/STAT3 signaling pathway in the angio-genesis of laryngeal cancer. Methods Serum levels of IL-17 and VEGF-A were determined in 70 laryngeal carci-noma patients using ELISA. The levels as well as clinicopathological features were analyzed. Western blot assay was used to detect the expressions of IL-17,IL-17R,JAK1/2,p-JAK1/2,STAT3,p-STAT3,VEGF-A,COX-2,MMP-9 in 70 cases of laryngeal carcinoma. Statistics methods were applied to analyze the results. Results Serum levels of both IL-17 and VEGF-A levels in patients were related to the degree of differentiation of laryngeal cancer, lymph node metastasis and TNM stage （P〈0. 05）, were unrelated to age,sex,primary tumor site. Western blot analysis showed that at different times in development of laryngeal cancer, with the deterioration of laryngeal cancer, IL-17, IL-17R expression gradually increased, JAK1/2,p-JAK1/2,STAT3,p-STAT3,VEGF-A,COX-2 and MMP-9 ex-pressions were also significantly increased. Conclusion IL-17 may play an important role in angiogenesis of laryn-geal cancer via regulating JAK/STAT3 signaling pathway.