白芍总苷对大鼠体外循环后肝损伤的保护作用

Effect of total glucosides of paeony on rat hepatic injury after cardiopulmonary bypass

目的:探讨白芍总苷对大鼠体外循环后肝损伤的保护作用及其机制.方法:将200只白鼠按照随机分组方式分为对照组、假手术组、低剂量组、中剂量组和高剂量组.利用显微外科器械和微型化体外循环环路设备,对除假手术组外大鼠进行体外循环.低、中、高剂量组大鼠在进行体外循环时分别在体外循环预充液中按照0.6、1.2和1.8 mg/kg加入白芍总苷.在体外循环2 h后,对各组大鼠血清转氨酶、总胆红素、前白蛋白、转铁蛋白、C反应蛋白、血清淀粉样蛋白A、肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、白介素-1β(interleukin-1β,IL-1β)、IL-6、血清生长激素(growth hormone,GH)、生长激素结合蛋白(growth hormone binding protein,GHBP)、胰岛素样生长因子-1(insulin-like growth factor-1,I G F-1)和胰岛素样生长因子结合蛋白-3(insulin-like growth factor binding protein-3,IGFBP-3)进行检测,肝细胞胆盐转运蛋白:钠/牛黄胆酸盐共转运子(Na+/taurocholate cotransporting polypeptide,NTCP)、胆盐输出泵(bile salt export pump,BSEP)、类法尼醇X受体(farnesoid X receptor,FXR)进行检测.结果:实验发现,对照组大鼠经过处理后血清转氨酶和总胆红素、前白蛋白、转铁蛋白、C反应蛋白、血清淀粉样蛋白A、T N F-α、I L-1β、I L-6显著高于假手术组(P<0.05),低、中、高剂量组上述指标显著低于对照组.GH、GHBP、IGF-1和IGFBP-3含量显著低于假手术组(P<0.05).而肝细胞胆盐转运蛋白NTCP、BSEP、FXR含量也显著低于假手术组(P<0.05).低、中、高剂量组显著高于对照组.且各剂量组在各项检查指标中也存在显著差异(P<0.05).结论:白芍总苷可以保护大鼠体外循环导致的肝损伤.

METHODS： Two hundred rats were randomly divided into a control group, a sham operation group, low-, medium- and high-dose TGP groups. Except the sham operation group, all other groups received CPB. In the low-,medium- and high-dose TGP treatment groups, 0.6, 1.2 and 1.8 mg/kg TGP was added into the priming solution, respectively. After 2 h of CPB, serum levels of transaminases, total bilirubin, prealbumin, transferrin, C-reactive protein, serum amyloid A, tumor necrosis factor alpha （TNF-α）, interleukin-1β （IL-1β）, IL-6, growth hormone （GH）, growth hormone binding protein （GHBP）, insulin-like growth factor-1 （IGF-1） and insulin-like growth factor binding protein-3 （IGFBP-3） were detected. Also, the expression of Na＊/taurocholate cotransporting polypeptide （NTCP）, bile salt export pump （BSEP） and farnesoid X receptor （FXR） in the liver was tested. RESULTS： Serum levels of glutamic pyruvic transaminase, aspartate transaminase, total bilirubin, prealbumin, transferrin, C-reactive protein, serum amyloid A protein, TNF-α, IL- 1β, and IL-6 were significantly higher in the control group after treatment than in the sham operation group （P 〈 0.05）; however, these parameters were significantly lower in the three treatment groups than in the control group （P 〈 0.05）. Serum levels of GH, GHBP, IGF-1 and IGFBP-3 as well as hepatic expression of NTCP, BSEP and FXR were significantly lower in the control group than in the sham operation group （P 〈 0.05）; however, these parameters were significantly higher in the three treatment groups than in the control group （P 〈 0.05）. There were significant differences in all parameters among the three treatment groups （P 〈 0.05）. CONCLUSION： TGP can protect against liver injury caused by CPB in rats.