上调DR-nm23表达对人结直肠癌细胞SW620生物学特性的影响

Effect of DR-nm23 overexpression on biological characteristics of human colorectal carcinoma SW620 cells

目的:探讨DR-nm23基因对人结直肠癌细胞SW620生物学特性的影响.方法:以无内源性DR-nm23表达组SW620、空白对照组SW620/mock和p GC-FU-DRnm23-GFP慢病毒转染组SW620/DR-nm23为实验对象,应用细胞体外实验及体内转移瘤模型检测分析DR-nm23转染前后对结直肠癌细胞增殖、运动、侵袭及转移能力的影响.结果:DR-nm23转染后能抑制SW620细胞的体外增殖能力,通过6 d连续比较,SW620/DRnm23、SW620/mock和SW620 3组细胞体外生长曲线(F=15.657,P=0.002)及平板克隆实验(F=45.476,P=0.003)差异有统计学意义.Transwell体外运动小室24 h穿过细胞数分别为SW620/DR-nm23组14.00±1.85,SW620/mock组18.00±2.01,SW620组17.00±1.98,3组差异有统计学意义(F=10.746,P=0.006).Boyden侵袭小室3组差异不显著.SW620/DRnm23组皮下成瘤能力(F=5.579,P=0.008)及肝转移能力较SW620/mock组及SW620组显著减弱.结论:外源性DR-nm23基因高表达具有抑制结直肠癌细胞SW620增殖、侵袭及转移的能力,因此可作为判断结直肠癌患者预后的潜在指标.

AIM： To investigate the effect of DR-nm23 overexpression on biological characteristics of human colorectal carcinoma SW620 cells. METHODS： SW620 cells were divided into three groups： non-endogenous expression （SW620） group, mock control （SW620/mock） group, and recombinant lentiviral expression vector pGC- FU-DR-nm23-GFP transfected （SW620/DR- nm23） group. Both in vitro cell experiments and in vivo xenograft tumor model assay were carried out to investigate the role of DR-nm23 in regulation of colorectal cancer cell proliferation, movement, invasion and metastasis. RESULTS： Induced overexpression of DR- nm23 in SW620 cells via lentiviral infection resulted in significant inhibition of cell proliferation as revealed by cell growth curve （F = 15.657, P = 0.002） and clonogenic assay （F = 45.476, P = 0.003） in vitro. Meanwhile, Transwell assay showed that the numbers of cells that passed the membrane in the SW620/DR-nm23 group, SW620/mock group and SW620 group were 14.00 ± 1.85, 18.00 ± 2.01, and 17.00 ±1.98, respectively, indicating that the migration ability was also significantly impaired in the SW620/DR-nm23 group （F = 10.746, P = 0.006）. There was no significant difference in the three groups in Boyden chamber assay. Besides, the growth rate （F = 5.579, P = 0.008） and liver metastasis rate of the SW620/DR-nm23 group were significantly reduced as compared with those of the SW620 or SW620/mock group in vivo. CONCLUSION： Overexpression of DR-nm23 may inhibit the invasive and metastatic capabilities of colorectal carcinoma SW620 cells. Thus, expression status of DR-nm23 may act as a potential prognostic factor in patients with colorectal cancer.