音乐干预对孕期应激仔鼠恐惧行为的影响及相关机制

Effect and Mechanism of Music Exposure on Fearful Behavior of Prenatal Stressed Rats

目的研究音乐干预对孕期应激仔鼠恐惧行为和杏仁核胃泌素释放肽受体（GRPR）表达的影响。方法 Wistar孕鼠16只随机分为应激组（n=8）和对照组（n=8）。应激组妊娠11-20 d每天将其置于窄小的固定器中并给予强光照射。对照组不给予刺激。应激组仔鼠出生后第1天起,予播放音乐（SM组）或相同分贝的白噪音（SW组）,SC组不给予刺激。生后60 d行防御退缩试验,免疫荧光组织化学和荧光实时定量PCR测定杏仁核GRPR及其mRNA表达。结果与其他两组比较,SM组仔鼠潜伏期显著缩短（P〈0.001）,出小室次数显著增多（P〈0.001）;杏仁核GRPR阳性细胞计数及mRNA表达显著增加（P〈0.001）。结论生后给予音乐干预可以纠正孕期应激仔鼠异常的恐惧行为,同时伴有杏仁核GRPR高表达。

Objective To investigate the effect of postnatal music exposure on fearful behavior of prenatal stressed rats in defensive withdrawal test and the expression of gastrin-releasing peptide receptor（GRPR） in the amygdala. Methods Wistar pregnant rats were divided in stress group（n=8） and control group（n=8）. The stress group were immobilized and bright lighted 45 min, 3 times a day, when gestation 11-20 days, while the control group without any stress. The new born rats from the stress group were exposed in music（SM group） or white noise（SW group） for 45 min twice a day since 1 day after born, and the rats from the control（SC） group left undisturbed. They were tested with defensive withdrawal test 60 days after birth, and the GRPR levels in the amygdala were measured with immunofluorescence and real-time PCR. Results The latency was shorter and the frequence out of the chamber was the most in the SM group of the defensive withdrawal test（P〈0.001）, while GRPR-positive cells and GRPR mRNA increased in amygdala（P〈0.001）. Conclusion Postnatal music exposure can counteract the abnormal behavior response of prenatal stressed offspring and accompany with increased expression of GRPR in the amygdala.