慢性前列腺感染病原体诊断中CD_(64)定量检测的临床评价

Clinical evaluation on the neutral granulocyte CD_(64) in quantitative detection of expressed prostatic secretions in chronic prostate infection

目的：观察和分析前列腺液（EPS ）中性粒细胞CD64定量检测，为慢性前列腺感染病原体诊断提供依据。方法选取2013年1月－2014年1月100例慢性前列腺炎（C P ）患者作为观察组，选取50名健康人作为对照组，采集两组受试者的EPS标本，对其中的病原体分布及中性粒细胞CD64表达量进行检测和比较。结果观察组患者的EPS标本中共检出病原体75株，其中检出细菌58株占77．34％，检出解脲脲支原体（Uu）13株、沙眼衣原体（Ct）4株，分别占17．33％和5．33％；Ct和Uu感染患者EPS中性粒细胞CD64表达量与对照组比较，差异无统计学意义，而观察组患者EPS中性粒细胞CD64表达量均显著高于对照组（q＝9．067～42．116，P＜0．05），其中，以模仿葡萄球菌和耳葡萄球菌感染患者EPS中性粒细胞CD64表达量最高，其次为溶血葡萄球菌、华纳葡萄球菌和粪肠球菌，最低为表皮葡萄球菌和人葡萄球菌人型亚种，以上差异均有统计学意义（ q＝7．034～31．208， P＜0．05）。结论革兰阳性菌是引发慢性前列腺感染的主要病原体，患者EPS中性粒细胞CD64表达量的升高提示细菌性感染的出现，可作为慢性前列腺感染病原体诊断的辅助指标。

OBJECTIVE To observe and analyze the neutral granulocyte CD64 quantitative detection of expressed prostatic secretious （EPS ） so as to provide reference for the pathogens diagnosis of chronic prostate infection . METHODS Totally 100 cases of the patients with chronic prostatitis （CP） from Jan .2013 to Jan .2014 were se‐lected as the observation group .Another 50 healthy people were selected as the control group .EPS of the objects in the two groups was collected ,and the distribution of pathogens and the expression of neutral granulocyte CD64 in EPS were detected and compared .RESULTS In EPS specimens of 100 patients with CP in this group ,75 strains of pathogens were detected ,and the detection rate was 75 .0% .Among them ,58 strains of bacteria were detec‐ted ,accounting for 77 .4% .Besides ,13 strains of Ureaplasma urealyticum （Uu） and 4 strains Chlamydia tra‐chomatis （Ct） were detected ,accounting for 17 .33% and 5 .33% respectively .The differences of the expression of neutral granulocyte CD64 of the objects in the control group and the objects with Ct or Uu infection were not signif‐icant .The expression of neutral granulocyte CD64 of the objects with bacterial infection was significantly higher than that in the control group （q=9 .067～42 .116 , P〈0 .05） ,in which ,the expression of neutral granulocyte CD64 of the patients with Staphylococcus imitatious and Staphylococcus auricular infection was the highest ,fol‐lowed by Staphylococcus epidermidis and Enterococcus f aecalis .S .epidermidis and human S .subspecies were the lowest ,the above differences were significant （q=7 .034～31 .208 , P〈0 .05） .CONCLUSION Gram‐positive bacteria are the main pathogens in EPS of patients with chronic prostate infection .The increased expression level of neutral granulocyte CD64 indicates the incidence of bacterial infection ,and can be used as an auxiliary index in the pathogen diagnosis of chronic prostate infection .