针刺对结肠吻合术后Cajal间质细胞修复环境中NO及NOS的影响

Effects of acupuncture on NO and NOS in restoration environment of interstitial cells of Cajal after colonic anastomosis

目的:探讨针刺促进Cajal间质细胞修复的机制。方法:30只SD大鼠随机分为空白组、模型组和针刺组,每组10只。模型组和针刺组予结肠吻合术制备结肠吻合术术后模型。造模成功后,针刺组穴取双侧足三针("后三里""三阴交""太冲")针刺,每次15min,每天1次,持续10d。模型组和空白组每天同一时间放于固定器中15min。测量各组大鼠小肠推进率,并取结肠组织用免疫组化法测c-kit表达,硝酸还原酶法测一氧化氮(NO)含量,L-精氨酸法测一氧化氮合酶(NOS)活性。结果:与空白组比较,模型组小肠推进率下降,NO含量升高,诱导型NOS(iNOS)活性增加,结构型NOS(cNOS)活性下降,总NOS(tNOS)活性增加,c-kit阳性Cajal间质细胞减少(均P<0.05);与模型组比较,针刺组小肠推进率提高,NO含量下降,iNOS活性降低,cNOS活性增加,tNOS活性降低,c-kit阳性Cajal间质细胞增加(均P<0.05)。结论:针刺能调节术后Cajal间质细胞修复环境中的NO含量和NOS活性,该作用可能参与了针刺促进Cajal细胞修复的过程。

Objective To explore the mechanism of acupuncture on promoting the restoration of interstitial cells of Cajal（ICCs）.Methods Thirty SD rats were randomly divided into a blank group,a model group and an acupuncture group,ten rats in each one.The rats in the model group and acupuncture group were treated with colonic anastomosis to establish the model.After successful establishment of the model,the rats in the acupuncture group were treated with acupuncture at bilateral＂Zusanli＂（ST 36）,＂Sanyinjiao＂（SP 6）and＂Taichong＂（LR 3）for 15 min,once a day for 10 days.Rats in the model group and blank group were put into the fixator for 15 min at the same time daily.The propulsive rate of small intestine was measured in each group.Colonic tissues were collected to detect c-kit expression by using immunohistochemistry.The nitricoxide（NO）content was measured by nitrate reductase method and nitric oxide synthase（NOS）activity was measured by method of L-arginine.Results Compared with the blank group,the propulsive rate of small intestine in the model group was decreased;NO content was increased;iNOS activity was elevated;cNOS activity was declined;total NOS（tNOS）activity was increased and the counting of c-kit positive ICCs was decreased（all P〈0.05）.Compared with the model group,the propulsive rate of small intestine in the acupuncture group was increased;NO content was decreased;iNOS activity was reduced;cNOS activity was elevated;NOS activity was decreased and the counting of c-kit positive ICCs was increased（all P〈0.05）.Conclusion Acupuncture can regulate NO content and NOS activity in postoperative restoration environment of ICCs,which may participate in the process of acupuncture promoting the restoration of ICCs.