白藜芦醇在大鼠体内的吸收代谢分析

Metabolism Analysis of Resveratrol and Piceid Contents in Rats

目的：高效液相色谱及气质联用法同时分析大鼠血浆和尿中白藜芦醇及白藜芦醇苷含量。方法：6w龄Wistar大鼠10只,适应性喂养1w后,分装于10个代谢笼中。实验前禁食10h,按体重灌胃给予大鼠50mg/kg白藜芦醇。高效液相色谱法检测大鼠血浆和尿中白藜芦醇及白藜芦醇苷的含量,并通过气质联用仪探索性分析不同时间血浆中代谢产物的种类。结果：白藜芦醇及白藜芦醇苷在0~10mg/L浓度范围内线性较好,其R2分别为0.9995及0.999 6。白藜芦醇灌饲1h后血浆中白藜芦醇浓度达到最大值,约4.79μg/m L,而白藜芦醇苷则到3h后才达到峰值,且其浓度达到白藜芦醇原型含量的5倍,约23.78μg/m L。白藜芦醇及其糖基化衍生物可在白藜芦醇摄入后24h内经尿液大量排泄到体外,排泄率超过摄入量的一半,其中原型占90%以上。气质联用分析进一步发现,机体摄入白藜芦醇3h后血浆中代谢物种类及含量均达到峰值。结论：白藜芦醇进入大鼠体内后在血浆中主要以衍生物形式存在,且代谢主要在摄入后前3h内,摄入后24h内主要以原型形式经尿液排出。

[Objective] To analyze the concentrations of resveratrol and its glucuronide conjugates in plasma and urine using HPLC and GC-MS. [Method] Totally 10 male Wistar rats were divided into 10 metabolic cages after acclimatization for 1 week. 50mg/kg resveratrol was given to rats by intragastric with normal diet under overnight fasting. The levels of resveratrol and its glueuronide conjugates were detected in the plasma using high performance liquid chromatography （ HPLC ） method with fluorescence detection （ FLD ）, and metabolites in the plasma were further determined with GC-MS. [Result] The maximum concentration of resveratrol in plasma was 4. 79μg/mL after consump- tion of resveratrol for 1 h, while the maximum concentration of piceid was up to 23.78μg/mL after 3h. Resveratrol and its glucuronide conju- gates were almost excreted by urine during 24h, and resveratrol was over 90% of all the excrement, more than 50% of intake. We further found the kinds and contents of metabolites were the peak after 3h in plasma with GC-MS method. [Conclusion] Pieeid was a major form of resveratrol existed in plasma, and mostly metabolized within the first 3h. Resveratrol was a primary constituent which excreted by urine dur- ing 24h.