蒙药珍宝丸对急性脊髓损伤大鼠的神经保护作用

Protective effects of Zhenbao pill on acute spinal cord injury

目的 探讨蒙药珍宝丸对急性脊髓损伤（acute spinal cord injury, ASCI）大鼠的神经保护作用及其机制.方法 按随机数字表法将64只Wistar大鼠分为模型组、珍宝丸组各32只.采用改良Allen＇s重物打击法制作胸T10水平急性脊髓不完全损伤大鼠模型.造模后30 min,珍宝丸组灌胃珍宝丸混悬液0.6 g/kg,模型组灌胃等体积生理盐水,1次/d.2组大鼠分别于造模后1、3、7、14 d进行行为学检测.于造模后1、3、7 d,2组分别随机抽取8只大鼠处死,分离脊髓,采用HE染色观察脊髓组织病理变化;采用硫代巴比妥酸分光光度计法测定脊髓丙二醛（MDA）水平;采用 TUNEL 染色观察细胞凋亡情况.结果 造模后1、3、7 d,与模型组比较,珍宝丸组大鼠脊髓组织MDA水平[分别为（15.12± 1.27）nmol/mg 比（19.71 ± 1.29）nmol/mg、（20.42 ± 1.33）nmol/mg 比（24.65 ± 1.36）nmol/mg、（10.46 ± 1.49）nmol/mg）比（15.46±1.44）nmol/mg）]、脊髓细胞凋亡率[（19.61±1.49）%比（26.61±1.52）%、（21.49± 1.37）%比（32.37±1.24）%、（13.04±1.30）%比（18.35±1.27）%]降低（P〈0.05）;造模后3、7、14 d,珍宝丸组 BBB 评分[（6.52±1.27）分比（2.63±1.27）分、（12.68±1.32）分比（6.17±1.34）分、（15.47±1.27）分比（11.57±1.29）分]高于模型组（P〈0.05）.结论 珍宝丸可改善大鼠ASCI后损伤段的脊髓组织病理损伤,降低MDA水平及神经细胞凋亡率,对大鼠ASCI后神经功能恢复有促进作用。

Objective To explore the protective effect of Zhenbao pill on acute spinal cord injury of rat and its possible mechanisms.Methods 64 Wistar rats were randomly divided into a injury group and a drug group, with 32 rats in each group, A T10 acute incomplete spinal cord injury model was produced by using modified Allen technique in the two groups,Zhenbao pill mixed suspension of 0.6 g/kg was given by gavage method daily in the drug group 30 min after moding, the same amount of saline was given in the injury group. Behavior detection were conducted in the first day, third day, and the seventh day after modeling to each 8 rats of the two groups. Spinal cord specimens was got centered about the damage points after rats were executed. histopathological changes were observed under HE staining, the MDA content were determined by thiobarbituric acid spectrophotometer and the apoptotic cells were marked by TUNEL method.Results After 1, 3, 7 d, compared with the injury group, the MDA level in the rat spinal cord tissue in the drug group （15.12 ± 1.27 nmol/mgvs.19.71 ± 1.29 nmol/mg, 20.42 ± 1.33 nmol/mgvs.24.65 ±1.36 nmol/mg, 10.46±1.49 nmol/mg vs.15.46 ± 1.44 nmol/mg） decreased（P〈0.05）; the apoptosis rate of drug groups （19.61% ± 1.49%vs.26.61% ±1.52%, 21.49% ± 1.37% vs.32.37% ± 1.24%, 13.04% ±1.30%vs.18.35% ± 1.27%）decreased （P〈0.05）; the BBB score of drug groups （6.52 ± 1.27vs. 2.63 ± 1.27）, （12.68 ± 1.32 vs.6.17 ± 1.34）, （15.47 ± 1.27 vs.11.57 ± 1.29） was higher than the injury group （P〈0.05）. Conclusion Zhenbao pill can significantly improve the histopathological examination,reduce the MDA content in the injured spinal cord specimen of the rat and the rate of neuronal apoptosis, which may promote recovery of neurologic function in the rat suffered from ASCI.