探讨植物性食品中有机氯农药残留量检测的前处理方法

Discussion Vegetable Foods Pretreatment Method Organochlorine Pesticide Residues Detected

目的：采用涡旋振荡提取法、超声波提取法和索式提取法测定菜干、茶叶和陈皮等植物性食物的有机氯农药残留量，比较提取率和回收率。方法称取粉碎样品与丙酮-石油醚混合均匀后，震荡提取上清液，重复3次，最后经吹氮仪浓缩后，加入硫酸或硫酸钠摇振、离心，取上清液进行色谱分析。结果采用以上三种提取方法检测的结果无差异，8种异构体回收率均较好，涡旋振荡提取法、超声波提取法和索式提取法的回收率分别为：85%～105%，82%～110%、84%～115%，回收率均满足检测要求，由于漩涡振荡法所耗时间更短，因此更推荐用此法作为植物性食物的有机氯农药残留的检测法。8种农药异构体在0.5～250 g/L的浓度范围线性良好，相关系数r为0.9984～1.0000，检测限可达0.10～0.65 g/L。结论涡旋震荡提取发因所耗溶剂最少，用时短，成本低等优点，可推广应用于植物性食品的有机氯农药残留的检测。

Objective Vortexed extraction method, determination of organochlorine pesticide residues in dried vegetables, tea and orange peel and other plant foods, compare the extraction rate and recovery ultrasonic extraction and Soxhlet extraction.MethodsWeigh crushed sample with acetone - petroleum ether mixed, shock supernatant was repeated three times, the last after blowing nitrogen concentrated, adding sulfuric acid or sodium shaken, centrifuged and the supernatant was chromatographed .Results The results of the above three extraction methods detect no difference, eight kinds of isomers of recovery are good, vortexed extraction, ultrasonic extraction recovery method and Soxhlet extraction were as follows： 85%~105%,82%~110%、84%~115%, recoveries are to meet testing requirements, since the vortex method a shorter amount of time spent, and therefore more recommend this method as a plant food organochlorine pesticide residue detection method. 8 pesticide isomers 0.5~250 g / L concentration range a good linear correlation coefficient r = 0.9984~1.000 0, the detection limit of 0.10~0.65 g/L. Conclusion The Vortex solvent extraction hair due to the consumption of the least, with time short, low cost, can be extended organochlorine pesticide residues in applied plant foods.