绵羊感染支原体后杀菌通透性增加蛋白(BPI)mRNA表达水平的变化

Change of bactericidal permeability increasing protein(BPI) mRNA expression level in the sheep infected with Mycoplasma ovipneumoniae

为了检测巴什拜羊和盘羊杂交羊在感染绵羊肺炎支原体(MO)前后杀菌通透性增加蛋白(BPI)表达水平的变化,对试验羊攻毒MO,在感染前(第0天)及感染后的第2、5、7、14及21天,颈静脉采血分离中性粒细胞,采用Real-time q PCR方法检测BPI的表达水平。结果显示,感染后第5天,2组BPI相对表达量升高。巴什拜羊BPI持续升高,杂交羊在第7天表达水平最高,在14-21天则逐渐降低。在第7天,巴什拜羊高于杂交羊(P<0.05),在第14-21天,巴什拜羊极显著高于杂交羊(P<0.01)。说明绵羊感染MO后初期BPI有明显升高趋势,在后期巴什拜羊和杂交羊的BPI变化有差异,这对研究支原体肺炎发病机理提供参考。

The aim of this study was to detect the dynamic change of bactericidal permeability increasing protein（ BPI） in Bashibai sheep（ BS） and Argali hybrid sheep（ AHS） infected with Mycoplasma ovipneumoniae（ MO）. All the sheep were artificially infected with MO.Neutrophilic granulocyte was seperated from jugular vein taken before infection（ 0d） and on the 2,5,7,14 and 21 days after infection（ dpi）,and relative expression level of BPI mRNA from neutrophils was detected by Real- time q PCR. The results showed that： On the5 dpi,BPI levels were higher than pre-infection. And BPI levels were increased with the duration of MO infection in BS. The level in AHS reached highest on the 7dpi,but declined gradually on the 14 th to 21 dpi. BPI level in BS were higher than that in AHS on the 7dpi（ P〈0. 05） and on the 14 th to 21dpi（ P〈0. 01; P〈0. 01）. It suggested that BPI level had an obviously elevating trend with the duration of MO infection,and there was a significant difference between the two species of sheep at the late stage of infection. This study provides a basis for investigation of MO pathogenesis.