摘要
目的探讨槲皮素对大鼠肝细胞同型半胱氨酸(homocysteine,Hcy)代谢的影响。方法实验1:培养的大鼠肝细胞分成2组,对照组和5μmol/L槲皮素组,分别用DMEM培养液和含有5μmol/L槲皮素的DMEM培养液培养;实验2(治疗性模型):培养的细胞分成4组:对照组、5μmol/L槲皮素组、50mmol/L蛋氨酸(methionine,Met)组和5μmol/L槲皮素+50mmol/L Met组,分别用空白DMEM培养液,含有5μmol/L槲皮素、50mmol/L Met以及5μmol/L槲皮素+50mmol/L Met的DMEM培养液培养24h,随后收集培养液检测Hcy;实验3(预防性模型):培养的细胞分成4组:对照组、5μmol/L槲皮素组、50mmol/L Met组和5μmol/L槲皮素+50 mmol/L Met组。在前23h,5μmol/L槲皮素组、5μmol/L槲皮素+50mmol/L Met组用含有5μmol/L槲皮素的DMEM培养液处理,其余两组用空白培养液处理;在实验结束前的1h,所有组均换液,分别用空白培养液,含有5μmol/L槲皮素、50 mmol/L Met和5μmol/L槲皮素+50mmol/L Met的培养液处理细胞1h。实验结束,收集各组培养液和肝细胞,检测培养液Hcy水平,肝细胞内谷胱甘肽(GSH)含量以及胱硫醚-β合成酶(CBS)活性和表达。结果在槲皮素处理组,槲皮素和甲基化槲皮素的含量分别为(36.74±0.05)μg/L和(31.57±0.03)μg/L。在实验2和实验3中,Met处理后Hcy水平显著增加,在实验3中槲皮素处理能够降低Hcy水平。实验3中槲皮素组及其槲皮素+Met处理组GSH含量增加,CBS活性显著增加。结论在Met负载大鼠肝细胞,槲皮素能通过促进转硫化途径来降低Hcy水平。
Objective To explore the effects of quercetin on homocysteine（Hcy）metabolism in rat hepatocytes. Methods Rat hepatocytes were cultured and treated with 5μmol/L quercetin,or 50mmol/L methionine（Met）,or both quercetin and Met,or DMEM alone as control for 24 h.Culture medium was collected and tested for Hcy level.Meanwhile,GSH and CBS levels in heptatocytes were also determined. Results The quercetin and methylated quercetin in hepatocytes were（36.74±0.05）μg/L and（31.57±0.03）μg/L（n=5）,respectively.Hcy release notably increased after exposure to Met,and decreased after quercetin treatment.The GSH level increased and the activity of CBS was enhanced by the combination of quercetin and Met treatment. Conclusions Quercetin may decrease the Hcy level by enhancing Hcy transsulfuration in rat hepatocytes exposed to Met.
出处
《中国预防医学杂志》
CAS
2015年第9期661-665,共5页
Chinese Preventive Medicine
基金
国家自然科学基金青年科学基金项目(30901184)
天津市应用基础与前沿技术研究计划(自然科学基金青年项目)(13JCQNJC11800)
