氧化低密度脂蛋白促进培养的人肾小球系膜细胞LOX-1表达

In vitro Study of LOX-1 Expression on Human Mesangial Cell Induced by Oxidized LDL

目的:研究氧化低密度脂蛋白(Ox-LDL)对人肾小球系膜细胞植物血凝素样受体(lectin-like oxidized low-density lipoprotein receptor,LOX-1)表达。方法:不同浓度的Ox-LDL和培养的人肾小球系膜细胞共孵育,应用Real-time PCR和Western Blot方法检测Ox-LDL对人肾小球系膜细胞LOX-1表达的影响。结果:Ox-LDL剂量和时间依赖性促进人肾小球系膜细胞LOX-1mRNA和蛋白表达。Ox-LDL 40μg/mL刺激细胞0-24小时,于12小时达峰值。Ox-LDL 10、20、40、60μg/mL分别作用于细胞12小时,40μg/mL组达到峰值,为基础值的3.73倍。Ox-LDL 40μg/mL刺激细胞0-24小时,LOX-1蛋白24小时达高峰,Ox-LDL 10、20、40、60μg/mL分别作用细胞24小时,40μg/mL组细胞LOX-1蛋白达到峰值,为基础值的1.81倍。结论:Ox-LDL在一定浓度范围内剂量和时间依赖性促进人肾小球系膜细胞LOX-1表达。

Objective： To observe whether the expression of lectin-like oxidized LDL receptor can be induced by Ox-LDL on human mesangial cell（HMC）. Methods： When cultured HMC stimulated by different doses of Ox-LDL, the levels of LOX-1 on HMC induced by Ox-LDL were examined using Real-time PCR and Western Blot. Results： Ox-LDL could induce LOX-1 mRNA and protein expression in a dose-dependent manner（10-40 μg/m L）. The peak level of LOX-1 mRNA reached after 12 h of stimulation and was as high as 3.73-fold as control. The up-regulation effect on LOX-1 protein reached peak after 24 h and was as high as 1.81-fold as control treated with 40 μg/ml of Ox-LDL. Conclusions： Ox-LDL can induce LOX-1 expression in a dose and time dependent manner.