转录因子WSTF对肺癌细胞增殖和侵袭的实验研究

WSTF Promotes Proliferation and Invasion of Lung Cancer Cells

目的:研究转录因子WSTF对肺癌细胞增殖和侵袭作用的影响。方法:采用慢病毒介导的基因转染方法建立A549细胞WSTF高表达细胞系A549-WSTF和空质粒对照细胞系A549-control。细胞增殖实验和克隆形成实验观察ING5过表达对肺癌A549细胞增殖能力的影响;Trans-well迁移实验和侵袭实验观察WSTF对肺癌细胞迁移和侵袭能力的影响。结果:Western blot验证A549-WSTF细胞WSTF蛋白水平显著高于对照细胞A549-control,P=0.0004。WSTF高表达明显促进了肺癌细胞的增殖能力(1-4天P值分别为0.002、0.0004、0.0002和3.21×10-5)和克隆形成能力(P=0.004);WSTF过表达还显著促进了肺癌细胞从trans-well小室迁移到下室的作用,其OD570值分别为0.626±0.013(A549-WSTF)和0.322±0.010(A549-control),P=2.37×10-5;WSTF还促进肺癌细胞穿透基质胶迁移到下室,其OD570值分别为0.600±0.027(A549-WSTF)和0.333±0.017(A549-control),P=0.0004。结论:WSTF可以促进肺癌细胞的增殖和侵袭能力而发挥促癌作用。

Objective： To investigate the effects of WSTF on the proliferative and invasive abilities of lung cancer cells. Methods：WSTF stably overexpressed A549 cell line（A549-WSTF） and control A549 cell line（A549-control） were established by lentiviral infection. Proliferation assay and colony formation assay were used to observe the effects of WSTF overexpression on the proliferative ability of lung cancer cells. Trans-well migration assay and invasion assay were performed to study the influence of WSTF on invasive capacity of lung cancer cells. Results： WSTF protein level in A549-WSTF cells was significantly higher than that of control cells（P=0.0004）. WSTF overexpression significantly promotes proliferation（P=0.002, 0.0004, 0.0002 and 3.21×10-5on day 1 to day 4） and colony formation abilities（P=0.004） of lung cancer A549 cells. In addition, WSTF overexpression increases the migration ability of lung cancer cells indicated by migrated cell OD570 value, with A549-WSTF（0.626±0.013） much higher than A549-control（0.322±0.010）（P=2.37×10-5）. Similar results were observed in trans-well invasion assay with the OD570 value of A549-WSTF（0.600±0.027） much higher than that of A549-control（0.333±0.017）（P=0.0004）. Conclusion： WSTF may act as an oncoprotein by promoting the proliferative and invasive abilities of lung cancer A549 cells, and thus could be a promising therapeutic target for the clinical treatment of lung cancer.