慢性睡眠限制状态下大鼠髁突软骨MMP-1,MMP-13表达的变化

Effects of Chronic Sleep Restriction on the MMP-1 and MMP-13 in the Condylar Cartilage of Rats

目的:探讨MMP-1,MMP-13在慢性睡眠限制引起大鼠髁突软骨结构变化中的表达变化及作用。方法:180只雄性Wistar大鼠随机分为3组(n=60):慢性睡眠限制组(CSR)、大平台组(LC)、笼养组(CON)。每组根据试验时间不同分别分为3个亚组(n=20):7天(7D)、14天(14D)、21天(21D)组。参考改良多平台法(MMPM)建立大鼠的慢性睡眠限制模型。通过HE染色观察大鼠髁突软骨的结构变化。通过免疫组化和实时定量PCR分别检测MMP-1和MMP-13的蛋白水平及m RNA水平的表达变化。结果:HE染色和扫描电镜结果显示,CSR组的大鼠髁突软骨出现了病理性的改变。与CON和LC组比较,CSR组MMP-1和MMP-13的m RNA转录和蛋白表达水平明显升高(P<0.05)。结论:慢性睡眠限制能够引起大鼠颞下颌关节髁突软骨的病理性变化。MMP-1和MMP-13的表达水平的变化可能在大鼠髁突软骨病理性改变中起关键作用。

Objective： To explore the changes and possible roles of MMP-1 and MMP-13 in the condylar cartilage of rats subjected to chronic sleep restriction（CSR）. Methods： One hundred and eighty male Wistar rats were randomly divided into three groups（n = 60 in each group）： chronic sleep restriction group（CSR）, large-platform control group（LC） and cage control group（CON）. Each group was divided into three subgroups（n = 20 in each subgroup） of three different time points（7, 14 and 21 days）, respectively. The modified multiple platform method was used to induce chronic sleep restriction. The condylar cartilage tissue histology was studied by staining with haematoxylin and eosin（HE）. The expression of MMP-1 and MMP-13 proteins and m RNAs was detected by immunohistochemistry and real-time polymerase chain reaction（RT-PCR）, respectively. Results： HE staining showed pathological alterations in the condylar cartilage following CSR. Compared with CON and LC groups, the m RNA and protein expression levels of MMP-1 and MMP-13 were upregulated after CSR（P〈0.05）. Conclusion： These data suggest that chronic sleep restriction may induce pathological alterations in the condylar cartilage of rats. Alterations in MMP-1 and MMP-13 may be play an important role in the mechanism by which CSR influences the condylar cartilage.