CMV-DNA与CMV-IgM对婴幼儿巨细胞感染的诊断价值

Diagnostic Value of CMV-DNA and CMV-Ig M Detection to Cytomegalovirus Infection in Infants

目的:研究尿液CMV-DNA联合血清CMV-Ig M检测对婴儿巨细胞感染的诊断价值。方法:选取2012年8月到2014年8月期间我院疑似巨细胞感染的婴幼儿70例,应用化学发光法检测血清CMV-Ig M,采用实时荧光定量PCR法(FQ-PCR)来检测尿液CMV-DNA,并比较经抗病毒治疗前和治疗后检测结果的变化。结果:单独检测尿CMV-DNA的阳性率为62.8%(44/70),单独检测血清CMV-Ig M的阳性率为55.7%(39/70),两者比较差异无统计学意义(P>0.05);两者联合检测的阳性率为77.1%(54/70),与两种方法单独检测比较差异具有统计学意义(P<0.05);经抗病毒治疗以后两种检测结果均显著降低,与治疗前比较差异具有统计学意义(P<0.05)。结论:尿液CMV-DNA联合血清CMV-Ig M检测能显著提高婴儿巨细胞感染的阳性率。

Objective： To study the diagnostic value of CMV-DNA in urine and serum CMV-Ig M detection to cytomegalovirus infection in infants. Methods： 70 cases suspected CMV infection in newborns were selected from our hospital during the period from August 2012 to August 2014, CMV-Ig M in serum were detected in chemiluminescence, CMV-DNA in urine were detected in real-time quantitative PCR（FQ-PCR）, before antiretroviral therapy and after treatment changed were compared. Results： Individually tested positive rate of CMV-DNA in the urine was 62.8%（44/70）, separate tested positive rate of CMV-Ig M in serum was 55.7%（39/70）, the difference was no statistically significant（P 〉0.05）; the positive rate of combined detection was 77.1%（54/70）, the difference was statistically significant compared with individually tested with both methods（P〈0.05）; two detection results were significantly lower after treatment by the anti-virus, the difference was statistically significant compared with before treatment（P〈0.05）. Conclusion： CMV-DNA in urine and serum CMV-Ig M detection can significantly improve the positive rate of cytomegalovirus infection in infants.