摘要
目的:探讨经氧化低密度脂蛋白(OX-LDL)刺激后,人脐静脉内皮细胞(PPAR-γ)表达的变化,以及瑞舒伐他汀对动脉粥样硬化的影响。方法:将实验标本随机分为2组,分为(OX-LDL)刺激组、瑞舒伐他汀干预组。应用RT-PCR及Western blot技术,观察OX-LDL诱导的人脐静脉内皮细胞PPAR-γ表达情况及瑞舒伐他汀对人脐静脉内皮细胞PPAR-γ表达的影响。结果:1)OX-LDL以时间及浓度依赖的方式降低了人脐静脉内皮细胞PPAR-γ的表达;2)瑞舒伐他汀可以逆转OX-LDL对人脐静脉内细胞的影响并可能与甲羟戊酸有关。结论:OX-LDL可降低人脐静脉内皮细胞PPAR-γ的表达。瑞舒伐他汀可以抑制OX-LDL诱导人脐静脉内皮细胞PPAR-γ表达的增强,从而可能抑制了OX-LDL信号通路介导的与炎症有关的血管损伤,延缓动脉粥样硬化的进程。
Objective： To observe the effect of Rosuvastatin on PPAR-γ in human umbilical vein endothelial cells. Methods：Human umbilical vein endothelial cells were isolated, and the effects of Rosuvastatin on PPAR-γ expression were evaluated by RT-PCR and Western blot. Results： The results demonstrated that concentration dependent and time dependent OX-LDL remarkably decreased the expression of the expression of PPAR-γ in human umbilical vein endothelial cells. However rosuvastatin can reverse the impact of OX-LDL, and the process may be associated with mevalonate. Conclusions： Rosuvastatin can control atherosclerosis process by stimulating PPAR-γ expression in human umbilical vein endothelial cells.
出处
《现代生物医学进展》
CAS
2015年第25期4956-4959,共4页
Progress in Modern Biomedicine
基金
黑龙江省自然科学基金项目(D201167)
