白及须根醇提物诱导肺腺癌细胞A549凋亡研究

Study the Effect of Alcohol Extract of Fibrous Root Part of Bletilla striata on Apoptosis of Lung Adenocarcinoma Cancer Cell Line of A549

目的研究白及须根醇提物(Alcohol extract of fibrous root part of Bletilla striata,AEFB)诱导肺腺癌细胞A549凋亡及相关作用机制。方法运用MTs、hoechst-PI双染及流式细胞术检测白及须根醇提物对A549细胞的增殖及凋亡影响;二维水平的细胞迁移检测白及须根醇提物对A549细胞转移的影响;RT-PCR法检测白及须根醇提物对肺腺癌细胞凋亡和转移相关基因bcl-2、bax、ICAM-1 m RNA的影响。结果白及须根醇提物能够抑制肺腺癌细胞的增殖,并且有浓度效应,随着药物浓度的提高,bcl-2、ICAM-1 m RNA表达下调,bax m RNA表达上调。结论白及须根醇提物能够明显的抑制肺腺癌细胞的生长作用,具有明显的促进肿瘤细胞凋亡作用和抑制肿瘤细胞的转移作用,其机制可能是通过下调bcl-2、ICAM-1及上调bax的表达相关。

Objective To investigate the inhibition of Alcohol extract of fibrous root part of Bletilla striata（AEFB） on proliferation and metastasis ability of A549 cells and to study their preliminary mechanism further. Methods MTs、Hoechst-PI staining and Flow cytometry were used to study the effects of proliferation,apoptosis by AEFB. The effect of AEFB on A549 cell migration in two dimensions,the influence of AEFB on the expression of bcl-2,bax,and ICAM-1 gene in A549 cell was evaluated by RT-PCR. Results We found that AEFB not only can inhibit the growth of A549 cell but also induce the cell apoptosis in dose and time dependent manner. With the increase of AEFB concentration,the expression of bcl-2,ICAM-1 gene were down-regulated and the expression of bax gene were up-regulated. Conclusion AEFB could inhibit the growth of A549 significantly,and has a significant role in promoting tumor cell apoptosis,and inhibit tumor cell migration. The mechanism may be related to decrease of bcl-2,ICAM-1 gene expression and increase bax gene expression.