黄瓜种质资源苗期耐低温性评价

Evaluation of Low Temperature Tolerance in Cucumber Germplasm

以43份不同类型的黄瓜品种(品系)为试验材料,在黄瓜苗期进行低温处理(昼/夜温度为12℃/9℃),以冷害指数作为鉴定指标对其耐低温性进行评价。最终鉴定出7份耐低温材料、10份低温敏感型材料。对黄瓜低温处理后的冷害指数(Y)与叶绿素降低率(X1)、可溶性蛋白增高率(X2)、MDA增高率(X3)、脯氨酸增高率(X4)、POD增高率(X5)进行相关性分析,并建立了鉴定黄瓜耐低温性的回归方程:Y=0.433+0.671X1+0.005X2+0.121X3-0.733X4+0.248X5。

Taking 43 cucumber varieties （ lines ） of different types as experimental material, this paper put the cucumber seedling under chilling stress （ day/night temperatures are 12 ℃/9℃ ）, and carried out evaluation on low temperature tolerance with cold injury index as identification indicator. Eventually, 7 varieties with low temperature tolerance, 10 varieties with low temperature sensitiveness were identified. The correlation analysis was carried out on cucumber cold injury index （Y） after low temperature treatment, the declined rate of chlorophyll （X1） and the increased rates of soluble protein （X2）, MDA （X3）, proline （ X4 ） and POD （ X5 ）. Then a regression equation for identifying cucumber low temperature tolerance was established ： Y=0.433＋0.671）（1＋0.005）（2＋0.121X3-O.733X4＋O. 248X5.