丹参酮ⅡA磺酸钠治疗爆炸致豚鼠急性肺损伤实验研究

Effect of tanshinone ⅡA sulfoacid sodium on NF-κB expression in lung tissue in the treatment of guinea pig with acute lung injury caused by explosion

目的采用不同浓度丹参酮IIA磺酸钠(STS)治疗火器伤引起豚鼠ALI时的肺保护作用。方法60只豚鼠随机分为正常对照组为N组、模型组为M组、生理盐水对照组为P组、低剂量STS治疗组(A组)、中剂量STS治疗组(B组)、高剂量STS治疗组(C组),每组10只,制作豚鼠ALI动物模型。造模0.5h后,采用免疫印迹法测定细胞核因子(NF-κB),用光学显微镜观察实验豚鼠病理变化情况,并及时记录其观察结果。结果病理学结果除N组外,造模12h各组动物的肺泡结构破坏,结构混乱,各种炎性细胞浸润,以M组最明显;用丹参酮IIA干预治疗各组中均有改善,以中等浓度STS干预治疗为最有效(P<0.05)。结论STS治疗对火器伤引起的豚鼠ALI具有保护作用,以STS中剂量保护肺作用最明显。

Objective To study the lung protective effect of tanshinone IIA sulfoacid sodium （ STS） in thetreatment of firearm injury-induced acute lung injury （ ALI） . Methods 60 guinea pigs were randomly divided into six groups：the normal control group （ Group N） , the acute lung injury model group （ Group M） , the physiological saline injection group （ Group P） , the low dose of STS treatment group （ Group A） , the middle dose of STS treatment group （Group B）, and the high dose of STS treatment group （Group C）. All groups had 10 guinea pigs to be in-duced with ALI. 0. 5 hours after modeling, the expression of NF-κB in lung tissues was detected by Western blot, and pathological changes in guinea pigs were observed by optical microscope. Results Except the N group, guinea pigs in the rest five groups showed alveolar structure damage and disorder, and inflammatory cell infiltration, with the most obvious in the group M. All groups improved after STS intervention, and the medium concentration of STS had the most effective effect （P〈0. 05）. Conclusion STS has the protective effect for guinea pigs with firearm injury-induced acute lung injury, and the middle dose has the best effect.