肿瘤坏死因子对于骨髓基质干细胞CXCR2表达的影响

Effect of TNF-α on the expression of CXCR2 in marrow stromal stem cells

目的观察肿瘤坏死因子(TNF-α)对于大鼠骨髓基质干细胞(MSCs)表达CXCR2的影响。方法采用有利于MSCs生长及增殖的全骨髓贴壁法分离及培养干细胞;成骨、成脂诱导检测其分化特性。采用TNF-α干预MSCs细胞培养。细胞分为:对照组、低剂量组(5 ng/m L)和高剂量组(10 ng/m L)。选取P3代细胞,培养10天后提取蛋白,Western blot分析MSCs表达CXCR2变化。结果所培养的MSCs成骨、成脂诱导后染色均阳性,证实所培养的即为MSCs细胞。提高培养液中TNF-α的浓度,则MSCs表达CXCR2的量显著增加,差异具有统计学意义。结论 TNF-α能够刺激MSCs表达CXCR2增加,这可能是急性肺损伤时,MSCs细胞动员和定植的机制之一。

Objective To evaluate the effect of TNF-α on the expression of chemokine receptors CXCR2 in mesenchymal stem cells （ MSCs） . Methods It used the approach of whole bone marrow adherent culture to isolate MSCs. Co-culture experiments were divided into three groups：the culture medium control group, the light concentra-tion cells （5 ng/mL） group, and the high concentration cells group stimulated by TNF-α. P3 cells of MSCs were cul-tured for 10 days, and then their proteins were derived. Immunoblotting was used to determine the expression of CX-CR2. Results 28 days after osteogenic induction of MSCs, calcium deposition was detected by Alizarin Red. 21 days after adipogenic differentiation,lipid droplets were detected by oil red. Compared with the culture medium con-trol group, the expression of CXCR2 increased significantly （P〈0. 05） in the light concentration cells group by west-ern-blot detection. Compared with the light concentration cells group, the expression of CXCR2 was significantly higher in the high concentration cells group （P〈0. 05）. Conclusion The expression of CXCR2 in MSCs increases stimulated by TNF-α, which is possibly one of the mechanism of MSCs imobilization and colonization in ALI.