30批中药饮片中检出5株非脱羧勒克菌

Five Strains of Leclercia adecarboxylata Detected in 30 Batches of Chinese Herbal Decoction Pieces

目的:对30批中药饮片中检出的5株非脱羧勒克菌进行分析,加强对该菌的认识。方法:按2015版《中国药典》征求意见稿的方法,检测30批中药饮片中需氧菌总数、霉菌和酵母菌总数及控制菌大肠埃希菌;将检出疑似的大肠埃希菌进行分离纯化、革兰染色和用Vitek2-compact全自动细菌鉴定仪鉴定;将检出的非脱羧勒克菌加入到相应的增菌培养基后,按2010版《中国药典》检测大肠埃希菌、大肠菌群和按2015版《中国药典》征求意见稿的方法检测耐胆盐革兰阴性菌。结果:30批中药饮片中检出5株非脱羧勒克菌。非脱羧勒克菌形态和生化反应结果与大肠埃希菌相似。将检出的非脱羧勒克菌加入到相应的增菌培养基后,按2010版《中国药典》检测,结果未检出大肠菌群;而按2015版《中国药典》征求意见稿的方法检测,检出耐胆盐革兰阴性菌。结论:非脱羧勒克菌与大肠埃希菌易于混淆,在鉴别时要注意区分。按2015版《中国药典》征求意见稿的方法检测非脱羧勒克菌,其被认为是耐胆盐革兰阴性菌。

Objective： To analyze 5 strains of Leclercia adecarboxylata detected in 30 batches of Chinese herbal decoction pieces, so as to have a better understanding of this bacteria. Methods： According to the method in the 2015 edition of Chinese Pharmacopoeia（draft）, the total aerobic microbial count and the total count of yeasts and molds were tested and Escherichia coli was controlled. The suspected Escherichia coli strains were separated and purifi ed, then were gram stained and identifi ed by the Vitek2-compact full-automatic instrument. After adding detected Leclercia adecarboxylata to the corresponding enrichment medium, Escherichia coli and coliform were detected according to the 2010 edition of Chinese Pharmacopoeia and bile salt-tolerant gram-negative bacteria were detected according to the 2015 edition of Chinese Pharmacopoeia（draft）. Results： Totally 5 strains of Leclercia adecarboxylata were detected in the 30 batches of Chinese herbal decoction pieces. The morphology and biochemical actions of Leclercia adecarboxylata were similar to Escherichia coli. After adding detected Leclercia adecarboxylata to the corresponding enrichment medium, Coliform was not found according to the 2010 edition of Chinese Pharmacopoeia and bile salt-tolerant gram-negative bacteria were detected as positive according to the 2015 edition of Chinese Pharmacopoeia（draft）. Conclusion： As Leclercia adecarboxylata is likely to be confused with Escherichia coli, attention should be paid when the two are to be distinguished. Leclercia adecarboxylatamay be identifi ed as bile salt-tolerant gram-negative bacteria when detecting according to the method in the 2015 edition of China Pharmacopoeia（draft）.