被污染的工业产品中常见微生物种类及耐药性分析

Distribution and drug resistance of common microorganisms from contaminated industrial products

为了工业产品中微生物污染治理及其防腐体系的构建提供科学依据,收集了被微生物污染的工业产品,并对其进行分离、鉴定和分类,再通过测定杀菌剂的最低抑菌浓度(MIC)来评估微生物的抗药水平。污染微生物中革兰氏阴性菌约占47.59%,革兰氏阳性菌约占32.62%,主要为芽孢杆菌属、假单胞菌属和伯克霍尔德氏菌;污染真菌约占19.8%,主要为链格孢霉属、曲霉属、青霉属。MIC结果表明,分离的洋葱伯克霍尔德氏菌对卡松、布罗波尔、DMDMH抗性均高于标准菌株,分离的铜绿假单胞菌、洋葱伯克霍尔德氏菌和黑曲霉对DMDMH的抗性均高于标准菌株。导致工业产品污染的微生物种类较多,应根据其种类、理化性状、防腐剂杀菌机理不同进行治理。

In order to provide the scientific basis for preventing the contamination of industrial products by mildewand bacteria and to construct its preservation system, the microorganisms from different type of industrial products wereisolated and classified. The minimum inhibitory concentrations for biocides were determined to estimate the microorganismresistance. It was found that the majority of isolates in the collection were Gram-negative bacteria （47.59%）, and theGram-positive bacteria （32.71% ）, including genera Pseudomonas, Bacillus and Burkholderia ; and fungi （ 19.8 % ） wereAspergillus, Alternaria and Penicillium. The value of MICs showed that Burkholderia cepacia to kathon, methyl 4-hydroxy-benzoate, bronopol, DMDMH was higher than that of the standard strain. And Pseudomonas aeruginosa, Burkholderiacepacia, and Aspergillus to DMDMH were higher than that of the standard strain. Industrial products were contaminated bymany microorganisms, so that their categories, physical and chemical properties should be comprehensively considered toconstruct the preservation system for preventing the contamination.