摘要
CRISPR/Cas是发现于细菌和古细菌基因组中的特殊结构,参与细菌和古细菌破坏噬菌体和外源质粒的免疫保护。科学家将II型CRISPR/Cas改造成为一个组装简便、高效和精准的基因组编辑工具,并迅速在动物、植物和微生物基因功能研究和遗传改造中获得广泛应用。本文介绍CRISPR/Cas9技术出现近两年来,在水稻、小麦、高粱、拟南芥、烟草、甜橙等植物中的研究情况,在此基础上对该技术的优点和需要进一步改进的地方提出了看法。
CRISPR/Cas is a specific gene structure found in the genome of bacteria and archaea, and is the immune system of bacteria and archaea involved in destroying phage and exogenous plasmids. CRISPR/Cas9, a convenient, precise and efficient genome editing technology, was developed according to the mechanism of type II CRISPR/Cas recently. From then on this technology has been broadly utilized to study gene functions and genetic modification of animal, plant and microorganism. The recent developments of CRISPR/Cas9 genome editing technology in rice, wheat, sorghum, Arabidopsis, tobacco and sweet orange, etc, were analyzed in detail in this paper. The advantages and further improvement aspects of this technology were also discussed at the end of this paper.
出处
《植物生理学报》
CAS
CSCD
北大核心
2015年第9期1351-1358,共8页
Plant Physiology Journal
基金
国家自然科学基金(30971709)
