摘要
目的了解东北林区啮齿动物感染多种蜱媒立克次体情况。方法采用PCR方法对吉林省集安、辽宁省宽甸林区采集的野鼠标本进行无形体、埃立克体16S r RNA和斑点热立克次体(SFGR)omp A基因片段检测。结果PCR检测鼠脾标本共132份,埃立克体、无形体、SFGR阳性率分别为14.39%、6.06%和10.61%。其中,集安66份,埃立克体、无形体、SFGR的阳性率均为7.58%;宽甸66份,埃立克体、无形体和SFGR的阳性率分别为21.21%、4.55%和13.64%,埃立克体感染率明显高于无形体和SFGR(χ2=8.058,P=0.018)。宽甸野鼠以埃立克体感染为主,阳性率明显高于集安(χ2=3.935,P=0.047)。不同鼠种埃立克体、无形体和SFGR的检测阳性率分别为黑线姬鼠13.79%、6.90%和13.79%,林姬鼠20.00%、4.00%和8.00%。132份野鼠标本中,埃立克体与无形体复合感染标本2份(1.52%),埃立克体与SFGR复合感染标本3份(2.27%),复合感染率为3.79%。结论调查地区野鼠中无形体、埃立克体、SFGR感染普遍,且存在复合感染,说明可能存在三者混合自然疫源地。
Objective To study the existence of human granulocytic anaplasmosis (HGA), human monocytic ehrlichiosis (HME) and tick-borne spotted fever (SF) in rodents in northeastern China. Methods Using polymerase chain reaction (PCR), Anaplasma (Ap), Ehrlichia (Ec) and spotted fever group rickettsiae (SFGR) were detected in rodent samples collected from Ji' an of Jilin province and kuandian of Liaoning province. Results In 132 wild rodent, 66 collected from Ji' an, Ap and Ee and SFGR were detected positive, with rates as 7.58%, respectively. 66 collected from kuandian, Ap and Ee and SFGR were detected positive, with rates as 21.21%, 4.55%, 13.64%, respectively. 3(2.27%) were eoinfections with Ec and Ap, while 2(1.52%) was eoinfections with Ee and SFGR. There was no coinfections of Ap and SFGR. Conclusion There exists natural foci of Ap, Ec and SFGR in the investigated area. Coinfections of Ec and Ap or Ec and SFGR did exist in rodents in Changbai mountain area, China.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2015年第5期467-470,共4页
Chinese Journal of Vector Biology and Control
基金
国家科技重大专项课题(2013ZX10004-203)
军队应用基础研究项目(AWS11L009)~~