江西省鼠类携带汉坦病毒基因特征研究

Analysis on genetic characteristics of Hantavirus from rodents in Jiangxi province

目的了解江西省鼠类汉坦病毒感染情况,分析汉坦病毒基因型别和基因特征。方法于2011年春秋季,在江西省高安市、安义县、新建县、上饶县、上高县、浮梁县室内外以夹夜法捕鼠取其肺,冷冻切片后采用直接免疫荧光法检测鼠肺中汉坦病毒抗原,提取阳性鼠肺标本RNA,采用特异性分型引物进行RT-PCR扩增,确定病毒型别;扩增汉坦病毒全S片段及部分M片段进行序列测定和基因分析。结果共捕获鼠类616只,41份免疫荧光阳性,鼠类汉坦病毒携带率为6.66%;对32份免疫荧光阳性鼠肺标本进行RT-PCR检测,其中10份为汉城型(SEOV型),13份为汉滩型(HTNV型),有9份标本未能分型;对7份标本序列测定和基因分析,结果显示4株HTNV型,3株病毒属SEOV型。结论江西地区鼠类携带汉坦病毒的型别主要为HTNV型和SEOV型,可能存在新亚型的汉坦病毒。

Objective To study the prevalence and the genetic characteristics of Hantavirus in the captured rodents in Jiangxi province. Methods Rodents were captured in the main epidemic areas, and Hantavirus antigens in rat lungs were detected by direct immunofluoreseenee assay. Total RNA were extracted from DFA positive lungs samples and amplified by using the special Hantavirus genotyping primers to genotype. The complete S segment and partial M segment were amplified by special primer; the products were cloned, and then sequenced and analyzed. Results Six hundred and sixteen rats were captured and Hantavirus antigens were identified in 41 lung samples by DFA. Ten SEOVs and thirteen HTNVs were found in 32 DFA positive lung samples by RT- PCR, Nine samples cannot be genotyped. Seven complete S segments were obtained by cloning and sequencing, the genetic and phylogenetic analysis of the complete S segment and partial M segment indicated that four viruses formed a distinct phylogenefic linage in HTNV clade; Three viruses belongs to SEOV. Conclusion The mainly epidemic Hantavirus were SEOV and HTNV in Jiangxi area, and there maybe presence of new Hantavirus subtype.