不同浓度锌的含氟矿化液对人恒前磨牙釉质脱矿影响的体外研究

Effect of Different Concentrations of Zinc on Demineralization of Human Premolar Enamel in vitro

目的研究含不同浓度微量元素锌(Zn)的含氟矿化液对离体恒前磨牙釉质脱矿的影响。方法采集和制备的牙釉质标本随机分为7组,将7组标本分别置于对照组(去离子水),不同浓度Zn(其中Zn的含量分别为0、10、50、100、150、200mg/L)的氟矿化液(含氟均为100mg/L)预处理8h,取出标本浸泡于酸蚀凝胶中,随后测定脱矿后8个时间点凝胶中的钙溶出量。结果含有不同浓度Zn的矿化液均有抑制牙釉质脱矿的能力(P=0.000),与对照组相比差异有统计学意义。同时含有Zn和F的比只含F的抑制脱矿能力增强,而且当Zn的含量为100mg/L时候,抑制脱矿的能力最强。结论 Zn和F共同作用能显著提高抑制釉质脱矿作用,且其抑制釉质脱矿作用与Zn浓度有相关性。

Objective To explore the zinc on demineralization of human premolar enamel in vitro with different concentrations of zinc containing fluorine mineralization fluid. Methods The human premolard enamel specimens were randomly divided into 7 groups： control group （distilled water） , different concentrations of strontium containing fluorine mineralization fluid group [ Zn^2＋ level （mg/L） of 0, 10,50,100,150,200 ]. After pre - treatment for 8h with the mentioned solutions respectively, the specimens were demineralized using acid gel at regular time intervals. The calcium contents released into the acid gel at regular time intervals were determined. Results Pre- treatment with zinc had significant influence on the contents of calcium released into the acid gel, compared with the control group. The to- tal contents of the released calcium in the control group were significantly higher than those in the zinc group. The effect of demineralized enamel specimens was strongest when the value of Zn2＋ at 100 mg/L. Conclusion The mineralization fluid solutions containing zinc and fluoride can enhance demineralization. The degree of demineralization may be related to Zn concentration.