有机相中脂肪酶催化转酯化反应动力学拆分左旋帕罗醇

Kinetic resolution of transesterification reaction of trans-paroxol catalyzed by lipase in organic media

研究了在有机相中脂肪酶催化转酯化反应动力学拆分左旋帕罗醇,考察了酶种类、溶剂、酰基供体、温度、底物与酰基供体摩尔比等因素对反应的影响。结果表明:以Novozym 435为催化剂,在30℃下,以乙腈为反应溶剂,乙酸乙烯酯为酰基供体,底物浓度40mmol/L及其与酰基供体摩尔比为1∶8时,反应8h后,底物转化率为48.1%,ees为53.3%,E值为6.20。

The kinetic resolution of transesterification reaction of trans-paroxol catalyzed by lipase was studied in organic media.The influence factors including enzyme source,solvent,acyl donor,temperature,substrate and the molar ratio of substrate with acyl donor were researched.The optimal conditions were obtained as following：Acetonitrile as solvent,viny acetate as acyl donor,concentration of substrate of 40mmol/L,the molar ratio substrate with acyl donor of 1∶8and reaction temperature 30℃.The conversion,the eesand the Evalue reached 48.1%,53.3%and 6.20 under the optimal conditions after 8h.