血浆miR-126在1型糖尿病患者中的变化及其临床意义

The changes and correlation analysis of plasma miR-126 level in T1DM patients

目的探讨T1DM患者血浆miR-126表达水平的变化及其临床意义。方法选取T1DM患者36例及健康对照(NGT)者34名,RT-PCR检测各组血浆miR-126,并分析其与年龄、BMI、高敏C反应蛋白(hsC-RP)、内皮型一氧化氮合酶(eNOS)、FPG、2hPG、HbA1c、FC-P、2hC-P、HDL-C、LDL-C、TG、TC的关系。结果与NGT组比较,T1DM组血浆miR-126表达下降[(1.65±0.61)vs(0.68±0.18),P<0.05]。相关分析显示,miR-126与HDL-C、FC-P、2hC-P及eNOS呈正相关(P<0.05或P<0.01),与LDL-C、TC、hsC-RP及年龄呈负相关(P<0.05或P<0.01);多元逐步回归分析显示,hsC-RP、FC-P、TC、年龄是miR-126的影响因素。结论 T1DM患者血浆miR-126呈低表达,可能与胰岛功能及内皮功能有关,hsC-RP及TC是miR-126的独立影响因素,miR-126可能参与了T1DM患者的炎症反应及脂质代谢异常,且miR-126水平随年龄增长而下降。

Objective To investigate the changes of plasma miR-126 expression level in patients with type 1diabetes（T1DM）. Methods The plasma miR-126 expression level was investigated in 36T1 DM patients and 34 healthy controls（NGT）by real-time fluorescent quantitative polymerase chain reaction（PCR）.The relationship between plasma miR-126 and clinical indicators such as age,BMI,hsCRP,eNOS,FPG,2hPG,HbA1 c,FC-P,2hC-P,HDL-C,LDL-C,TG and TC was analyzed by correlation analysis.The main influencing factors for miR-126 were analyzed by multiple stepwise regression analysis.Results The miR-126 expression level was significantly decreased in T1 DM group than that in NGT group[（1.65±0.61）vs（0.68±0.18）,P〈0.05].Correlation analysis showed that miR-126 was positively correlated with HDL-C,FCP,2hC-P and eNOS（P〈0.05 or P〈0.01）,and negatively correlated with LDL-C,TC,hsC-RP and age（P〈0.05 or P〈0.01）.Multiple stepwise regression analysis showed that hsC-RP,FC-P,TC and age were independent influencing factors for miR-126. Conclusion The expression level of plasma miR-126 is lower in T1 DM group than in NC group,which is possibly associated with islet and endothelial functions.hsC-RP and TC are independent influencing factors for miR-126,which may be involved in the inflammatory reaction and lipid metabolism disorder in T1 DM patients.With age,the expression level of miR-126 may decrease.