黄芩提取物对H22移植瘤小鼠的影响

Effects of Scutellaria Radix Extracts on H22 Transplanted Tumor in Mice

目的探讨黄芩提取物对肝癌H22模型小鼠的抑瘤作用及其可能的作用机制。方法将H22瘤株接种于小鼠,建立移植瘤模型。造模成功后随机分为模型组、5-Fu组及黄芩提取物低、中、高剂量组,另取12只正常小鼠作为对照组。各给药组给予相应药物灌胃,观察给药后小鼠体质量,计算肿瘤抑制率、胸腺及脾脏指数,分光光度法检测血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,单细胞凝胶电泳检测外周血淋巴细胞DNA损伤。结果黄芩提取物中、高剂量对肿瘤生长有显著抑制作用。与5-Fu组比较,黄芩提取物中、高剂量组能显著提高小鼠体质量、胸腺和脾脏指数,提高血清T-AOC、SOD活性,降低MDA含量,减轻外周血淋巴细胞DNA损伤。结论黄芩提取物具有明显的抗肿瘤作用,其作用机制可能与减轻肿瘤对机体正常组织细胞的伤害、修复或增强小鼠的免疫功能,以及提高机体抗氧化损伤能力有关。

Objective To study the antitumor effects of Scutellaria Radix extracts on H22 transplanted tumor in mice and its possible mechanism. Methods The H22 transplanted mouse models were established by inoculating H22 to mice. 60 mice were randomly divided into model group, 5-Fu group, low-, medium- and high-dose Scutellaria Radix extracts groups, with 12 normal mice as control group. All administration groups received gavage with relevant medicine. And then the body weight change, tumor growth inhibitory rate, and spleen and thymus indexes were calculated;the T-AOC and activities of SOD, and MDA content in serum were detected by spectrophotometer;DNA damage of peripheral blood lymphocytes was observed by single cell gel electrophoresis. Results Medium- and high-dose Scutellaria Radix extracts can significantly inhibit the growth of transplanted tumor. Compared with 5-Fu group, medium- and high-dose Scutellaria Radix extracts groups notably increased the body weight, spleen and thymus indexes of mice, promoted T-AOC and activities of SOD, and decreased MDA content in serum, as well as notably reduced DNA damage of peripheral blood lymphocytes. Conclusion Scutellaria Radix extracts have obvious antitumor effects on H22 transplanted tumor in mice and the possible mechanisms may be due to lightening the damage of normal tissue and cell from tumor, enhancing immunologic function and improving antioxidant capability of H22 bearing mice.