补肾中药对肾虚质大鼠脑源性神经营养因子表达的影响

Effect of Kidney-supplementing TCM on Brain-derived Neurotrophic Factor Expression of Rats with Kidney-Deficiency Constitution

目的观察补肾中药对肾虚质大鼠脑源性神经营养因子(BDNF)表达的影响,探讨生物体海马区神经元功能活动与肾藏志理论相关性。方法采用"猫吓鼠"经典造模法造成先天不足加后天失养复合型肾虚质模型仔鼠,分为模型组、左归丸组、右归丸组,空白组产自正常孕鼠,选用补肾中药进行调理。检测大鼠脑质量、肾质量、脑重指数及海马区BDNF的表达。结果与空白组比较,肾虚质大鼠脑质量、肾质量、脑重指数和BDNF光密度值降低;镜下观察海马区出现片状水肿,神经细胞数目减少、结构松散。补肾中药干预后,脑质量、肾质量、脑重指数及BDNF光密度值得到一定程度回升;大脑皮质包括海马区神经细胞增多,细胞结构致密且层次清楚,无明显水肿区。结论大鼠海马BDNF表达变化与肾藏志理论具有相关性;补肾中药可显著改善肾虚质大鼠脑功能衰退状况。

Objective To observe the effects of kidney-supplementing TCM on the brain-derived neurotrophic factor(BDNF) expression of rats with kidney-deficiency constitution;To discuss the correlation between functional activity of neurons in hippocampus with the theory of kidney storing aspiration. Methods Composite rat models with offspring deficiency and acquired dystrophy were built through the method of 'cats scare rats', and then were divided into model group, Zuogui Pill group, Yougui Pill group, and blank group in which offspring came from normal pregnant rats. After the treatment with kidney-supplementing TCM, weight of brain and kidney, brain weight indexes and the BDNF expression were detected. Results Compared with the blank group, the quality of brain, kidney and brain weight indexes, BDNF expression decteased of rats in model group were lower;flaky edema appeared in hippocampus observed by microscope;the number of nerve cells decreased and loosely structured. After the treatment of kidney-supplementing TCM, the weight of brain and kidney and brain weight indexes recovered to some extent;BDNF expression increased;nerve cells in cerebral cortexincluding hippocampus increased;the cell structure compacted and the level cleared as well as no edema. Conclusion The BDNF expression has correlation with the theory of kidney storing aspiration. Kidney-supplementing TCM can improve brain function and significantly improve declining situation of brain function of rats with kidney-deficiency constitution.