甘草饮片及其配方颗粒中甘草苷和甘草酸含量比较

Content Comparison of Liquiritin and Glycyrrhizic Acid Between Glycyrrhizae Radix et Rhizoma Decoction Pieces and Its Formula Granules

目的比较甘草饮片及其配方颗粒中甘草苷和甘草酸的含量差异。方法采用高效液相色谱-梯度洗脱法。色谱柱:Waters Atlantis T3(4.6 mm×150 mm,3μm);流动相A为乙腈,流动相B为0.1%磷酸溶液;流速:0.8 m L/min;检测波长:237 nm;柱温:30℃。测定甘草饮片及其配方颗粒中甘草苷和甘草酸的含量,比较其含量差异。结果配方颗粒中甘草苷和甘草酸的含量均低于甘草饮片的含量,与所标示的浓缩倍数不符。结论配方颗粒中甘草苷和甘草酸的含量与甘草饮片差异较大,配方颗粒的生产工艺需要改进。

Objective To compare the contents of liquiritin and glycyrrhizic acid between Glycyrrhizae Radix et Rhizoma decoction pieces and its formula granules. Methods HPLC method was used with Waters Atlantis T3 column(4.6 mm × 150 mm, 3 μm), flow rate of 0.8 m L/min, detection wavelength of 237 nm, and column temperature at 30 ℃. The mobile phase was acetonitrile-0.1% phosphoric acid solution gradient elution system. The contents of liquiritin and glycyrrhizic acid of Glycyrrhizae Radix et Rhizoma decoction pieces and its formula granules were determined and compared. Results The contents of liquiritin and glycyrrhizic acid in formula granules were less than that in the decoction pieces of Glycyrrhizae Radix et Rhizoma, which was not in conformity with marked concentration multiple. Conclusion The contents of liquiritin and glycyrrhizic acid in formula granules is greatly different with Glycyrrhizae Radix et Rhizoma decoction pieces. The production process of the formula granules needs to be improved.