六味地黄汤对IgA肾病模型大鼠转化生长因子-β1和补体调节蛋白CR1表达的影响

Effects of Liuwei Dihuang Decoction on Expressions of Transforming Growth Factor β1 and Complement Regulatory Protein CR1 of IgA Nephropathy Rats

目的观察六味地黄汤对IgA肾病模型大鼠转化生长因子-β1(TGF-β1)和补体调节蛋白CR1表达的影响,并探讨其作用机制。方法 40只SD大鼠适应性喂养1周,按完全随机方法分为正常组、模型组、雷公藤组、六味地黄汤组。采用复合方法建立IgA肾病模型,成模后各给药组给予相应药物灌胃,连续4周。检测各组大鼠尿常规、尿红细胞计数和24 h尿蛋白,取血检测肌酐、尿素氮、血清总蛋白、血清白蛋白,取肾组织HE、Masson染色观察大鼠肾脏病理结构,免疫组化检测大鼠肾脏组织TGF-β1和CR1的表达。结果与模型组比较,六味地黄汤组模型大鼠蛋白尿、血尿及肾脏纤维化减轻(P<0.05),TGF-β1表达下降、CR1表达上调(P<0.05)。结论六味地黄汤可能通过降低TGF-β1的生成,促进CR1的表达,延缓IgA肾病模型大鼠肾脏纤维化。

Objective To discuss the effects of Liuwei Dihuang Decoction on expressions of TGF-β1 and complement regulatory protein CR1 of the renal on IgA nephropathy rats. Methods Forty SD rats were randomized into normal group, model group, tripterygium glycosides group and Liuwei Dihuang Decoction group after adaptive feeding for one week. Complex method was used to establish IgA nephropathy model. All administration groups were given relevant medicine for gavage for 4 weeks;urine routine, the number of urine erythrocyte and 24 h urine protein were detected;creatinine, urea nitrogen, total serum protein and serum alburin were detected from blood;the renal pathological changes were observed under light microscope by HE and Masson staining;the expressions of TGF-β1 and CR1 were detected by immunohistochemistry. Results Compared with model group, Liuwei Dihuang Decoction could significantly decrease the proteinuria and hematuria levels and renal fibrosis changes of IgA nephropathy model rats, reduce the expression of TGF-β1, and raise the expression of CR1(P <0.05). Conclusion Liuwei Dihuang Decoction can delay the renal fibrosis in IgA nephropathy rats through reducing the degree of TGF-β1 and promoting the expression of CR1.