IL-2基因启动子区域T-384G基因多态性与中国汉族人肠易激综合征的关系

Association between IL-2 T-384G Polymorphisms and Irritable Bowel Syndrome in Chinese Han Population

目的探讨IL-2基因启动子区域T-384G基因多态性与肠易激综合征(irritable bowel syndrome,IBS)及其血清IL-2水平的关系。方法采用病例-对照研究方法纳入IBS患者(IBS组,n=213)及年龄、性别相匹配的健康人群(对照组,n=213)。以聚合酶链反应-限制性内切酶片段长度(PCR-RFLP)方法分析2组IL-2基因启动子区域T-384G基因多态性。以酶联免疫吸附方法(ELISA)测定2组血清IL-2水平。结果 IBS组及对照组TT、TG及GG基因型分布频数分别为77、100、36及115、86、12。IBS组GG基因分布显著高于对照组(P<0.05)。GG基因型人群血清IL-2水平显著高于TT+TG(P<0.05)。结论 GG基因型是IBS易患基因,可能与其具有较高的IL-2表达水平有关。

Objective To explore the relationships of promoter region T-384G polymorphisms of interleukin-2(IL-2)gene to irritable bowel syndrome(IBS)and serum IL-2 levels.Methods A case-control study was carried out,and 213 IBS patients and 213 age- and sex-matched healthy controls were recruited in this study.IL-2 T-384G polymorphisms and serum IL-2 levels were de-termined by PCR-RFLP and ELISA,respectively.Results The frequencies of TT,TG and GG genotypes were,respectively,77,100 and 36 in IBS group,and 115,86 and 12 in control group. Compared with control group,the frequency of GG genotype significantly increased in IBS group (P <0.05).Compared with TT+TG carriers,serum IL-2 levels significantly increased in GG car-riers(P <0.05).Conclusion GG genotype,a susceptible gene of IBS,is associated with higher levels of serum IL-2.