复方浙贝颗粒联合三苯氧胺对LCC2移植瘤相关蛋白表达影响

Effect of Compound Zhe Bei Granul Combined with Tamoxifen on the Expression of Associated Protein of LCC2 Xenografts

目的:研究复方浙贝颗粒(CZBG)联合三苯氧胺(TAM)对LCC2移植瘤组织相关蛋白HER-2、GST-π、Caspase3表达的影响。方法:用耐TAM的人乳腺癌细胞系LCC2建立小鼠移植瘤模型。实验分模型对照组,TAM组,阿霉素(ADM)+TAM组,中剂量CZBG+TAM组,ADM+中剂量CZBG+TAM组,采用免疫组化法分别检测各组HER-2、GST-π、Caspase3的表达差异。结果:1与TAM组相比,中剂量CZBG+TAM组、ADM+中剂量CZBG+TAM组能下调HER-2表达(P<0.05)。2与模型对照组相比,各治疗组GST-π表达均有所下降(P<0.05);各治疗组之间的GST-π表达无统计学意义(P>0.05)。3与模型对照组相比,中剂量CZBG+TAM组和ADM+中剂量CZBG+TAM组均能上调Caspase3表达(P<0.05);中剂量CZBG+TAM组与模型对照组、TAM组、ADM+TAM组比较,Caspase3的表达均有统计学意义(P<0.05)。结论:中剂量CZBG能够下调乳腺癌LCC2细胞移植瘤组织的HER-2表达,提高Caspase3活性,而对GST-π表达无明显影响。

Objective： To study the expression of associated protein HER-2,GST- π,Caspase3 of LCC2 xenografts treated by Compound Zhe Bei granul（CZBG） combined with Tamoxifen（TAM）. Methods： Xenograft model was made by using human breast cancer cells LCC2 resistant to TAM. Then the mice were randomly divided into model control group, simple TAM group, adriamycin（ADM）＋TAM group, middle dose of CZBG＋TAM group and ADM＋middle dose of CZBG＋TAM group. Differential expression of HER-2,GST- π, Caspase3 of each group was detected by using immunohistochemistry, respectively. Results： 1 Middle dose of CZBG＋TAM group and ADM＋middle dose of CZBG＋TAM group could down-regulate the expression of HER-2 compared with simple TAM group,which had statistical differences（P〈0.05）. 2The expression of GST-π in each treatment group declined compared with model control group（P〈0.05）. The expression of GST-π between treatment groups had no significant differences（P〉0.05）. 3The expression of Caspase3 in middle dose of CZBG＋TAM group and ADM＋middle dose of CZBG＋TAM group was increased significantly compared with model control group, which had statistical differences（P〈0.05）. And the expression of Caspase3 in middle dose of CZBG＋TAM group also had statistical differences compared with simple TAM group and ADM＋TAM group（P〈0.05）. Conclusions： Middle dose of CZBG can down-regulate HER-2 expression of LCC2 xenografts, improve Caspase3 activity, but has no effect on GST-π expression.